Ghanta V K, Hiramoto N S, Soong S J, Hiramoto R N
Department of Biology, University of Alabama, Birmingham 35294.
Mech Ageing Dev. 1991 May;58(2-3):245-54. doi: 10.1016/0047-6374(91)90096-i.
Studies of the effect of short-term, intense treatment with thymic hormone on mitogen response, cytotoxicity to EL-4 lymphoma and natural killer cell (NK) activity was investigated Balb/c nude mice (about 12-16-week-old) were treated 5 times per week for 3 weeks with: Facteur Thymic Serique (FTS) and Thymopentin (TP5, Thymopoietin 32-36) at 1 microgram and 10 ng; TM4 1 ng (an enzyme resistant variant of FTS); Thymosin Fraction V (TF5), 10 and 1 microgram; and 0.1 ml saline, and killed 2 days after the last treatment. The animals were monitored for changes in weight, hematocrit, peripheral blood lymphocyte (PBL) and spleen mitogen response. Additional groups of nude mice were immunized with 1 x 10(7) 5000 R irradiated EL-4 cells 10 days before sacrifice and tested for the presence of cytotoxic T-lymphocytes (CTL). The results show that weight and hematocrit were similar among the groups. Treatment with FTS significantly elevated the number of PBL. Spleen stimulation in mice treated with 1 microgram TP5 was depressed to mitogen concanavalin A (ConA) and lipopolysaccharide (LPS) stimulation. The phytohemagglutinin (PHA) response was not different among the treatment groups. The PBL mitogen response to ConA and LPS was generally increased over saline control in the hormone treated groups but was not statistically significant. The PHA response was only slightly elevated. No CTL was generated in nude mice in any of the groups. However, there was a statistically significant general depression of NK activity in all of the hormone treated animals compared with saline. The results indicate that the basic differentiation defect of the T-cells of nude mice cannot be restored to full functional activity by short-term treatment.
研究了胸腺激素短期强化治疗对丝裂原反应、对EL-4淋巴瘤的细胞毒性以及自然杀伤细胞（NK）活性的影响。选用约12 - 16周龄的Balb/c裸鼠，每周治疗5次，共3周，治疗药物及剂量如下：胸腺血清因子（FTS）和胸腺五肽（TP5，胸腺生成素32 - 36），剂量分别为1微克和10纳克；TM4 1纳克（FTS的一种酶抗性变体）；胸腺素组分V（TF5），剂量分别为10微克和1微克；以及0.1毫升生理盐水。在最后一次治疗后2天处死动物。监测动物体重、血细胞比容、外周血淋巴细胞（PBL）和脾脏丝裂原反应的变化。另外几组裸鼠在处死前10天用1×10⁷个经5000伦琴照射的EL-4细胞进行免疫，并检测细胞毒性T淋巴细胞（CTL）的存在情况。结果显示，各组之间体重和血细胞比容相似。FTS治疗显著提高了PBL数量。用1微克TP5治疗的小鼠脾脏对丝裂原刀豆球蛋白A（ConA）和脂多糖（LPS）刺激的反应受到抑制。各治疗组之间植物血凝素（PHA）反应无差异。激素治疗组中PBL对ConA和LPS的丝裂原反应总体上比生理盐水对照组有所增加，但无统计学意义。PHA反应仅略有升高。所有组的裸鼠均未产生CTL。然而，与生理盐水组相比，所有激素治疗动物的NK活性均有统计学意义的普遍降低。结果表明，短期治疗不能使裸鼠T细胞的基本分化缺陷恢复到完全功能活性状态。
