Ghanta V K, Noble P J, Brown M E, Cox P J, Hiramoto N S, Hiramoto R N
Mech Ageing Dev. 1983 Jul-Aug;22(3-4):309-19. doi: 10.1016/0047-6374(83)90085-4.
C57B1/6NNia mice 1, 12, and 24 months old showed loss of cellular-mediated cytotoxicity with aging. Treatment of the three age groups with different thymic hormone preparations effected their cellular mediated cytotoxicity differently. When cytotoxicity of the thymic hormone treated groups was compared to that of the physiological saline treated group, 1-month-old mice treated with serum thymic factor (FTS) at 1 microgram/mouse and 10 ng/mouse had significantly higher activity, and lower to similar activities at 12 and 24 months; TP5 (active fragment of thymopoietin) at 1 microgram and 10 ng caused significantly higher activity in 1-month-old mice, and lower to higher and significantly lower to similar activity at 12 and 24 months, respectively; TM4 (an analogue of TP5) at 1 ng showed significantly depressed activity in 1-month-old mice, and significantly enhanced activity in 12- and 24-month-old mice; thymosin at 10 micrograms and 1 microgram had slightly lower, but not significant, depression at 1 month, similar activities at 12 months and significantly depressed to higher activity at 24 months. Unimmunized control mice showed significant protection in the 12-month-old mice in comparison to 1- and 24-month-old mice. Different hormone preparations showed age- and dose-dependent effects on the ability of spleen cells to kill P815 mastocytoma. Partial restoration of cytotoxicity was observed in 24-month-old mice treated with FTS, TP5 and thymosin fraction V.
1月龄、12月龄和24月龄的C57B1/6NNia小鼠随着年龄增长表现出细胞介导的细胞毒性丧失。用不同胸腺激素制剂处理这三个年龄组，对其细胞介导的细胞毒性产生不同影响。将胸腺激素处理组的细胞毒性与生理盐水处理组的细胞毒性进行比较时，1月龄小鼠以1微克/只和10纳克/只的剂量注射血清胸腺因子（FTS）后，其活性显著更高，而在12月龄和24月龄时活性较低至相似；1微克和10纳克的胸腺生成素活性片段（TP5）使1月龄小鼠的活性显著更高，而在12月龄时活性较低至更高，在24月龄时活性显著更低至相似；1纳克的TP5类似物（TM4）使1月龄小鼠的活性显著降低，而使12月龄和24月龄小鼠的活性显著增强；10微克和1微克的胸腺素在1月龄时活性略有降低但不显著，在12月龄时活性相似，在24月龄时活性显著降低至高活性。与1月龄和24月龄小鼠相比，未免疫的对照小鼠在12月龄小鼠中表现出显著的保护作用。不同的激素制剂对脾细胞杀伤P815肥大细胞瘤的能力呈现出年龄和剂量依赖性效应。用FTS、TP5和胸腺素组分V处理24月龄小鼠后，观察到细胞毒性有部分恢复。
