Klionsky Daniel J, Elazar Zvulun, Seglen Per O, Rubinsztein David C
Autophagy. 2008 Oct;4(7):849-50. doi: 10.4161/auto.6845. Epub 2008 Oct 22.
Bafilomycin A(1) is a specific inhibitor of the vacuolar type H(+)-ATPase (V-ATPase) in cells, and inhibits the acidification of organelles containing this enzyme, such as lysosomes and endosomes. Recently, while editing and reviewing chapters on autophagy for Methods in Enzymology, we noticed repeated references to the effect of bafilomycin A(1) in blocking the fusion of autophagosomes with lysosomes. Of course we have seen this in various research papers as well, but reading this routinely in chapters written by various people over a short period of time really caused this to stand out. Every one of these chapters referred to the paper by Yamamoto et al. In that paper, treatment with 100 nM bafilomycin A(1) for 1 h blocks the fusion of autophagosomes with lysosomes in the rat hepatoma H-4-II-E cell line, based on data from electron microscopy. However, data from one of our labs noted an apparently different result in a relatively recent manuscript. Therefore, we decided to look into this more carefully.
巴弗洛霉素A1是细胞中液泡型H(+) -ATP酶（V-ATP酶）的特异性抑制剂，可抑制含有该酶的细胞器（如溶酶体和内体）的酸化。最近，在编辑和审阅《酶学方法》中关于自噬的章节时，我们注意到多次提及巴弗洛霉素A1在阻断自噬体与溶酶体融合方面的作用。当然，我们在各种研究论文中也看到过这一点，但在短时间内由不同的人撰写的章节中经常读到这一点，确实使其格外引人注目。这些章节中的每一篇都引用了山本等人的论文。在那篇论文中，基于电子显微镜数据，用100 nM巴弗洛霉素A1处理1小时可阻断大鼠肝癌H-4-II-E细胞系中自噬体与溶酶体的融合。然而，我们其中一个实验室的数据在最近的一篇手稿中显示出明显不同的结果。因此，我们决定更仔细地研究这个问题。
