Urabe T
First Department of Surgery, Okayama University Medical School, Japan.
Nihon Geka Gakkai Zasshi. 1991 May;92(5):543-50.
Lymphokine activated killer (LAK) cells can destroy not only tumor cells but also syngeneic regenerating liver cells. This study was started to determine the effect of passive transfer of LAK cells on liver regeneration after partial hepatectomy. C3H mice were received 70% hepatectomy and LAK cells were injected intravenously at a dose of 5 X 10(7) cells/body. After 36 hours, 3H-thymidine uptake into the residual liver was measured. LAK cells transferred group showed 31% suppression compared with control group. In vitro, 24 hours addition of LAK cells to the primary culture of regenerating liver cells caused 97% suppression of 3H-thymidine uptake at effector to target ratio, 50/1. Then we examined the effects of IL-2 administration on liver regeneration. Though IL-2 showed no effect on cultured liver cells, intraperitoneal administration of IL-2 after hepatectomy at a dose of 1 X 10(4)u/body 5 times every 8 hours brought 38% suppression of 3H-thymidine uptake of the residual liver. Cyclosporine A, which can suppress the IL-2 production of lymphocytes, promoted liver regeneration 45% over the control at a dose of 10 mg/kg. These results suggest that LAK cells could regulate liver regeneration.
淋巴因子激活的杀伤细胞（LAK细胞）不仅能破坏肿瘤细胞，还能破坏同基因的再生肝细胞。本研究旨在确定被动转移LAK细胞对部分肝切除术后肝脏再生的影响。对C3H小鼠进行70%肝切除术，并以5×10⁷个细胞/只的剂量静脉注射LAK细胞。36小时后，测量³H-胸腺嘧啶核苷掺入残余肝脏的情况。LAK细胞转移组与对照组相比显示出31%的抑制率。在体外，以效应细胞与靶细胞比例50/1向再生肝细胞原代培养物中添加LAK细胞24小时，导致³H-胸腺嘧啶核苷掺入抑制97%。然后我们研究了给予白细胞介素-2（IL-2）对肝脏再生的影响。尽管IL-2对培养的肝细胞没有影响，但肝切除术后以1×10⁴单位/只的剂量每8小时腹腔注射IL-2 5次，使残余肝脏的³H-胸腺嘧啶核苷掺入受到38%的抑制。环孢素A能抑制淋巴细胞产生IL-2，以10mg/kg的剂量使用时，促进肝脏再生比对照组高45%。这些结果表明LAK细胞可以调节肝脏再生。
