Ono M, Tanaka N, Orita K
First Department of Surgery, Okayama University Medical School, Japan.
Jpn J Surg. 1989 Nov;19(6):726-37. doi: 10.1007/BF02471724.
Spleen cells that are cultured with interleukin 2 for as short a time as 4 days develop the ability to lyse syngeneic natural killer-resistant tumor cells but not to lyse syngeneic lymphoblasts. When mice were subjected to partial hepatectomy (HEP), the spleen cells exhibited not only an augmentation of natural killer activity, but also an augmentation of in vitro induction of lymphokine activated killer (LAK) cells. Furthermore, the LAK cells exhibited lytic activities against syngeneic lectin-induced lymphoblasts and regenerating liver cells. The sensitivity of regenerating liver cells to lysis by LAK cells was detected as early as one day after HEP, and continued until day 14. Analysis by cell depletion techniques using monoclonal antibodies and complement, as well as discontinuous gradient sedimentation, indicated that the LAK cells activated by HEP were Thy-1+, Lyt-2+, asialo GM1+ and Lyt-1-, lymphocytes with a low density. After the intravenous (i.v.) administration of anti-asialo GM1 before HEP, the in vitro induction of LAK cells was remarkably inhibited.
用白细胞介素2培养仅4天的脾细胞就获得了裂解同基因自然杀伤抗性肿瘤细胞的能力，但不能裂解同基因淋巴母细胞。当对小鼠进行部分肝切除术（HEP）时，脾细胞不仅自然杀伤活性增强，而且体外诱导淋巴因子激活的杀伤细胞（LAK细胞）的能力也增强。此外，LAK细胞对同基因凝集素诱导的淋巴母细胞和再生肝细胞具有裂解活性。早在肝切除术后1天就检测到再生肝细胞对LAK细胞裂解的敏感性，并持续到第14天。使用单克隆抗体和补体的细胞清除技术以及不连续梯度沉降分析表明，由HEP激活的LAK细胞是Thy-1 +、Lyt-2 +、无唾液酸GM1 +且Lyt-1 -的低密度淋巴细胞。在肝切除术前静脉内（i.v.）给予抗无唾液酸GM1后，LAK细胞的体外诱导受到显著抑制。
