Tanaka N, Tatemoto A, Urabe T, Ono M, Hizuta A, Naomoto Y, Gotoh K, Moreira L F, Orita K
First Department of Surgery, Okayama University Medical School, Japan.
Acta Med Okayama. 1993 Feb;47(1):21-8. doi: 10.18926/AMO/31607.
Lymphokine activated killer (LAK) cells can destroy not only tumor cells but also syngeneic liver cells. In this study, the effects of passive transfer of LAK cells on liver regeneration were examined by the 3H-thymidine uptake and bromodeoxyuridine (BrdU) labeling methods after resection of 70% of the volume of the liver. LAK cells were infused 12h after hepatectomy and the effects on regeneration of liver cells were examined 36 h later. The transfusion of LAK cells induced significant inhibition of liver regeneration at a dose of 5-10 x 10(7) cells. Neuraminidase treatment of lymphocytes is desirable to enhance the selective entrapment of LAK cells into the liver. When LAK cells were treated with neuraminidase (0.5 units/ml), and transfused into hepatectomized mice, more potent suppression of liver regeneration was induced in comparison with the same dose of LAK cells. The intraperitoneal injection of recombinant interleukin 2 (rIL-2) after partial hepatectomy also inhibited the regeneration of remnant liver. From these results, lymphocytes such as LAK cells appear to regulate liver regeneration.
淋巴因子激活的杀伤（LAK）细胞不仅能破坏肿瘤细胞，还能破坏同基因的肝细胞。在本研究中，通过3H-胸腺嘧啶核苷摄取和溴脱氧尿苷（BrdU）标记法，在切除70%肝体积后，检测LAK细胞被动转移对肝再生的影响。肝切除术后12小时输注LAK细胞，并在36小时后检测其对肝细胞再生的影响。输注剂量为5 - 10×10（7）个细胞时，LAK细胞可显著抑制肝再生。用神经氨酸酶处理淋巴细胞有助于增强LAK细胞在肝脏中的选择性滞留。当用神经氨酸酶（0.5单位/毫升）处理LAK细胞并输注到肝切除小鼠体内时，与相同剂量的LAK细胞相比，对肝再生的抑制作用更强。部分肝切除术后腹腔注射重组白细胞介素2（rIL-2）也会抑制残余肝脏的再生。从这些结果来看，诸如LAK细胞之类的淋巴细胞似乎对肝再生具有调节作用。
