Faraco V, Pezzella C, Miele A, Giardina P, Sannia G
Department of Organic Chemistry and Biochemistry, University of Naples Federico II, Complesso Universitario Monte S. Angelo, via Cintia 4, Naples, Italy.
Biodegradation. 2009 Apr;20(2):209-20. doi: 10.1007/s10532-008-9214-2. Epub 2008 Aug 30.
The effect of Phanerochaete chrysosporium and Pleurotus ostreatus whole cells and their ligninolytic enzymes on models of colored industrial wastewaters was evaluated. Models of acid, direct and reactive dye wastewaters from textile industry have been defined on the basis of discharged amounts, economic relevance and representativeness of chemical structures of the contained dyes. Phanerochaete chrysosporium provided an effective decolourization of direct dye wastewater model, reaching about 45% decolourization in only 1 day of treatment, and about 90% decolourization within 7 days, whilst P. ostreatus was able to decolorize and detoxify acid dye wastewater model providing 40% decolourization in only 1 day, and 60% in 7 days. P. ostreatus growth conditions that induce laccase production (up to 130,000 U/l) were identified, and extra-cellular enzyme mixtures, with known laccase isoenzyme composition, were produced and used in wastewater models decolourization. The mixtures decolorized and detoxified the acid dye wastewater model, suggesting laccases as the main agents of wastewater decolourization by P. ostreatus. A laccase mixture was immobilized by entrapment in Cu-alginate beads, and the immobilized enzymes were shown to be effective in batch decolourization, even after 15 stepwise additions of dye for a total exposure of about 1 month.
评估了黄孢原毛平革菌和糙皮侧耳的全细胞及其木质素分解酶对有色工业废水模型的影响。基于纺织工业排放的酸性、直接和活性染料废水的量、经济相关性以及所含染料化学结构的代表性，确定了相应的废水模型。黄孢原毛平革菌能有效使直接染料废水模型脱色，在处理仅1天时脱色率约为45%，7天内脱色率约为90%；而糙皮侧耳能够使酸性染料废水模型脱色并解毒，在仅1天时脱色率为40%，7天时为60%。确定了诱导糙皮侧耳产生漆酶（高达130,000 U/l）的生长条件，并制备了具有已知漆酶同工酶组成的细胞外酶混合物，用于废水模型的脱色。这些混合物使酸性染料废水模型脱色并解毒，表明漆酶是糙皮侧耳使废水脱色的主要因素。通过包埋在铜藻酸盐珠中固定漆酶混合物，并且即使在逐步添加染料15次、总暴露约1个月后，固定化酶在分批脱色中仍显示出有效性。
