Yamaoka N, Tagawa Y, Miyashita K, Ayabe H, Kawahara K, Uchiyama Y, Tomita M
First Department of Surgery, Nagasaki University, School of Medicine, Japan.
Nihon Geka Gakkai Zasshi. 1991 May;92(5):598-602.
Flow cytometric cellular DNA-RNA content analysis by acridine orange staining was conducted on surgical fresh specimens of primary lung carcinomas from 66 patients (31 squamous cell carcinomas, 34 adenocarcinomas and 1 large cell carcinoma). The frequency of aneuploid tumors was 84.6% among the tumors. RNA content (RNA Index) in the DNA aneuploid tumor much more significantly (p less than 0.05) increased than the DNA diploid tumor. Tumor doubling time in the DNA aneuploid tumor was significantly (p less than 0.05) shorter than in the DNA diploid tumor. In the patients with lung cancers that recurred within 1 years, recurrence of the DNA aneuploid tumor was higher than the DNA diploid tumor. It is evident from the above results that proliferative activity in the DNA aneuploid tumor increases much more than in the DNA diploid tumor. This in turn may induce early recurrence in patients with lung cancer.
采用吖啶橙染色法对66例原发性肺癌手术新鲜标本（31例鳞状细胞癌、34例腺癌和1例大细胞癌）进行流式细胞术细胞DNA - RNA含量分析。肿瘤中异倍体肿瘤的发生率为84.6%。DNA异倍体肿瘤中的RNA含量（RNA指数）比DNA二倍体肿瘤显著增加（p小于0.05）。DNA异倍体肿瘤的肿瘤倍增时间比DNA二倍体肿瘤显著缩短（p小于0.05）。在1年内复发的肺癌患者中，DNA异倍体肿瘤的复发率高于DNA二倍体肿瘤。从上述结果可以明显看出，DNA异倍体肿瘤中的增殖活性比DNA二倍体肿瘤增加得多。这反过来可能导致肺癌患者早期复发。
