Effluent organic matter (EfOM) contributes significantly to organic fouling of reverse osmosis (RO) membranes in advanced wastewater reclamation. In this study, the effect of feed solution chemistry (solution pH and Ca(2+) concentration) on the fouling of RO membranes by octanoic acid-selected to represent fatty acids in EfOM-is investigated. Crossflow fouling experiments demonstrate that RO membrane fouling is much more significant at solution pH below the pK(a) of the octanoic acid (pK(a)=4.9) than at an elevated pH. Octanoic acid permeates across the membranes more readily at solution pH below its pK(a) than at elevated pH. At pH below the octanoic acid pK(a), fouling behavior is not affected by calcium ions, whereas at elevated pH, the rate of flux decline decreases with higher calcium ion concentration. The effect of calcium on the fouling behavior was further verified from foulant-foulant adhesion forces, determined by atomic force microscopy (AFM) force measurements under solution chemistries identical to those of the crossflow fouling experiments. To investigate the implications of octanoic acid fouling for wastewater reclamation, the effect of octanoic acid on membrane fouling by a combination of organic foulants in the presence of calcium ions is studied. At a solution chemistry simulating that of typical wastewater effluents, the addition of octanoic acid to a feed solution containing alginate, bovine serum albumin, and Suwannee River natural organic matter, does not enhance membrane fouling behavior. This observation could be attributed to the significant contribution of the alginate-calcium complexes within the fouling layer to the total membrane resistance.