Ameur Fatma Zohra, Mehedi Nabila, Soler Rivas Cristina, Gonzalez Antonio, Kheroua Omar, Saidi Djamel
Laboratory of Physiology of Nutrition and Food Security, University Oran1 Ahmed BenBella, Oran, Algeria.
2Department of Production and Characterization of Novel Foods, CIAL-Research Institute in Food Science (UAM-CSIC), Madrid, Spain.
Toxicol Res. 2019 Nov 21;36(2):159-166. doi: 10.1007/s43188-019-00023-3. eCollection 2020 Apr.
Tartrazine (E102) is a synthetic food coloring, which belongs to the class of mono azo dyes and is known to cause numerous health problems. The current research aimed to evaluate the effect of this food dye on the enzymatic activity of amylase, lipase and proteases after a subchronic ingestion in Swiss mice. Additionally, an in vitro digestion model was used to highlight the relationship between the probable toxicity of tartrazine and the nature of the food ingested. The results show that there were no adverse effects of tartrazine on the body weight gain, and on amylase or lipase activities. However, in the high dose of tartrazine (0.05%) group, a significant decrease in trypsin and chymotrypsin enzymatic activities were observed. Regarding the in vitro digestion model, our findings show that there were no changes in the trypsin and chymotrypsin enzymatic activities either using 7.5 or 75 mg of tartrazine mixed with rice, butter or milk. We conclude that excessive consumption of tartrazine appears to alter the enzymatic activity of proteases in vivo which may have deleterious consequences on digestion. Even thought the dose close to the acceptable daily intake does not affect those activities, a strict control of tartrazine dose in high-consumption foods especially among children is an indispensable task.
柠檬黄（E102）是一种合成食用色素，属于单偶氮染料类别，已知会引发众多健康问题。当前研究旨在评估在瑞士小鼠亚慢性摄入这种食用色素后，其对淀粉酶、脂肪酶和蛋白酶的酶活性的影响。此外，还使用了体外消化模型来突出柠檬黄可能的毒性与所摄入食物性质之间的关系。结果表明，柠檬黄对体重增加以及淀粉酶或脂肪酶活性没有不良影响。然而，在高剂量柠檬黄（0.05%）组中，观察到胰蛋白酶和糜蛋白酶的酶活性显著降低。关于体外消化模型，我们的研究结果表明，将7.5毫克或75毫克柠檬黄与大米、黄油或牛奶混合使用时，胰蛋白酶和糜蛋白酶的酶活性均未发生变化。我们得出结论，过量食用柠檬黄似乎会在体内改变蛋白酶的酶活性，这可能对消化产生有害影响。尽管接近每日可接受摄入量的剂量不会影响这些活性，但严格控制高消费食品中柠檬黄的剂量，尤其是在儿童中，是一项不可或缺的任务。
