Upadhyaya Lakshmeesha, Semsarilar Mona, Quemener Damien, Fernández-Pacheco Rodrigo, Martinez Gema, Coelhoso Isabel M, Nunes Suzana P, Crespo João G, Mallada Reyes, Portugal Carla A M
Advanced Membranes and Porous Materials Center (AMPM), Biological and Environmental Science and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), 23955-6900 Thuwal, Saudi Arabia.
Institut Européen des Membranes, IEM UMR 5635, Univ Montpellier, ENSCM, CNRS, 34070 Montpellier, France.
Membranes (Basel). 2021 Feb 2;11(2):105. doi: 10.3390/membranes11020105.
In this study, we report the impact of the magnetic field on protein permeability through magnetic-responsive, block copolymer, nanocomposite membranes with hydrophilic and hydrophobic characters. The hydrophilic nanocomposite membranes were composed of spherical polymeric nanoparticles (NPs) synthesized through polymerization-induced self-assembly (PISA) with iron oxide NPs coated with quaternized poly(2-dimethylamino)ethyl methacrylate. The hydrophobic nanocomposite membranes were prepared via nonsolvent-induced phase separation (NIPS) containing poly (methacrylic acid) and meso-2,3-dimercaptosuccinic acid-coated superparamagnetic nanoparticles (SPNPs). The permeation experiments were carried out using bovine serum albumin (BSA) as the model solute, in the absence of the magnetic field and under permanent and cyclic magnetic field conditions OFF/ON (strategy 1) and ON/OFF (strategy 2). It was observed that the magnetic field led to a lower reduction in the permeate fluxes of magnetic-responsive membranes during BSA permeation, regardless of the magnetic field strategy used, than that obtained in the absence of the magnetic field. Nevertheless, a comparative analysis of the effect caused by the two cyclic magnetic field strategies showed that strategy 2 allowed for a lower reduction of the original permeate fluxes during BSA permeation and higher protein sieving coefficients. Overall, these novel magneto-responsive block copolymer nanocomposite membranes proved to be competent in mitigating biofouling phenomena in bioseparation processes.
在本研究中，我们报告了磁场对蛋白质透过具有亲水性和疏水性的磁响应嵌段共聚物纳米复合膜的渗透性的影响。亲水性纳米复合膜由通过聚合诱导自组装（PISA）合成的球形聚合物纳米颗粒（NPs）组成，其中的氧化铁纳米颗粒涂覆有季铵化的聚（甲基丙烯酸2-二甲基氨基乙酯）。疏水性纳米复合膜是通过非溶剂诱导相分离（NIPS）制备的，其中含有聚（甲基丙烯酸）和中-2,3-二巯基琥珀酸包覆的超顺磁性纳米颗粒（SPNPs）。渗透实验以牛血清白蛋白（BSA）作为模型溶质，在无磁场以及在永久和循环磁场条件下进行，磁场条件为关/开（策略1）和开/关（策略2）。结果观察到，无论使用何种磁场策略，在BSA渗透过程中，磁场导致磁响应膜的渗透通量降低程度低于无磁场时的情况。然而，对两种循环磁场策略所产生的效果进行的比较分析表明，策略2在BSA渗透过程中使原始渗透通量的降低程度更小，并且具有更高的蛋白质筛分系数。总体而言，这些新型的磁响应嵌段共聚物纳米复合膜在减轻生物分离过程中的生物污染现象方面表现出色。
