J Chromatogr. 1976 Dec 22;129:31-9. doi: 10.1016/s0021-9673(00)87765-1.
The determination of a number of impurities in neohesperidin dihydrochalcone is described, as well as the determination of neohesperidin dihydrochalcone in food products. A reversed-phase chromatographic system is used, with octadecyltrichloro-silane-treated silica gel as chemically bonded stationary phase and methanol-water as mobile phase; a detailed procedure for preparing the packing material is given, and the dependence of the amount of bonded stationary phase on the humidity of the silica gel before the bonding reaction is shown. The glass column was packed at 350 atm in a steel pressure vessel, with use of a slurry-packing technique; details are given of the pressure vessel and of the packing method. Chromatograms are reproduced to show the performance of such columns in this type of analysis.
本文描述了新橙皮苷二氢查耳酮中多种杂质的测定方法，以及食品中新橙皮苷二氢查耳酮的测定方法。采用反相色谱系统，以十八烷基三氯硅烷处理的硅胶作为化学键合固定相，甲醇 - 水作为流动相；给出了制备填充材料的详细步骤，并展示了键合固定相的量与键合反应前硅胶湿度的关系。玻璃柱在钢制压力容器中于350个大气压下采用匀浆填充技术进行填充；详细介绍了压力容器和填充方法。重现了色谱图以展示此类柱在该类型分析中的性能。
