Liquid chromatography of neohesperidin dihydrochalcone.

The determination of a number of impurities in neohesperidin dihydrochalcone is described, as well as the determination of neohesperidin dihydrochalcone in food products. A reversed-phase chromatographic system is used, with octadecyltrichloro-silane-treated silica gel as chemically bonded stationary phase and methanol-water as mobile phase; a detailed procedure for preparing the packing material is given, and the dependence of the amount of bonded stationary phase on the humidity of the silica gel before the bonding reaction is shown. The glass column was packed at 350 atm in a steel pressure vessel, with use of a slurry-packing technique; details are given of the pressure vessel and of the packing method. Chromatograms are reproduced to show the performance of such columns in this type of analysis.