Kang Yuejun, Wu Xudong, Wang Yao-Nan, Li Dongqing
Department of Mechanical Engineering, Vanderbilt University, Nashville, TN 37235, USA.
Anal Chim Acta. 2008 Sep 19;626(1):97-103. doi: 10.1016/j.aca.2008.07.043. Epub 2008 Aug 3.
A fluorescence-activated particle counting and sorting system is developed for lab-on-a-chip applications. This system integrates the microfluidic chip, fluorescence excitation and detection, electronic power switch control, and optical visualization. The automatic sorting function is achieved by electrokinetic flow switching, which is triggered by a pre-set fluorescent threshold. A direct current electric pulse is generated to dispense the fluorescent particles to the collection reservoir. A user-friendly software interface is developed for automatic real-time counting, sorting and visualization. The design of the disposable microfluidic chip is simple and easy for integration. This system represents a promising prototype for development of affordable and portable flow cytometric instruments.
一种用于芯片实验室应用的荧光激活粒子计数与分选系统被开发出来。该系统集成了微流控芯片、荧光激发与检测、电子电源开关控制以及光学可视化。自动分选功能通过电动流切换来实现，其由预设的荧光阈值触发。产生直流电脉冲以将荧光粒子分配到收集容器中。开发了一个用户友好的软件界面用于自动实时计数、分选和可视化。一次性微流控芯片的设计简单且易于集成。该系统是开发经济实惠且便携的流式细胞仪的一个有前景的原型。
