Do Jaephil, Lee Sehwan, Han Jungyup, Kai Junhai, Hong Chien-Chong, Gao Chuan, Nevin Joseph H, Beaucage Gregory, Ahn Chong H
Electrical and Computer Engineering, University of Cincinnati, Cincinnati, OH 45221, USA.
Lab Chip. 2008 Dec;8(12):2113-20. doi: 10.1039/b811169c. Epub 2008 Oct 23.
This paper presents the development of an easy-to-handle and disposable clinical diagnostic lab-on-a-chip using fully integrated plastic microfluidic components, which has the sampling/identifying capability to make fast and reliable measurements of metabolic parameters from human whole blood. A smart and functional lab-on-a-chip cartridge, which incorporates a full on-chip auto-calibration function for in the field applications, has been developed, and then fully characterized using a portable analyzer (3 (1/4)''x 5''x 1'') with multi-analyte detection capability. In addition, several new approaches in realizing smart and functional lab-on-a-chips on polymer have been adopted, which include the pinch valve for automatic fluidic sealing, a by-pass channel as the sampling indicator, and a robust connector design for long analyzer lifetimes. Metabolic parameters such as glucose, lactate, and partial oxygen from human whole blood have been successfully measured using the functional polymer lab-on-a-chips and the portable analyzer developed in this work.
本文介绍了一种使用完全集成的塑料微流控组件开发的易于操作且一次性使用的临床诊断芯片实验室，其具有采样/识别能力，能够对人类全血中的代谢参数进行快速可靠的测量。开发了一种智能且功能齐全的芯片实验室试剂盒，该试剂盒具有用于现场应用的全芯片自动校准功能，然后使用具有多分析物检测能力的便携式分析仪（3（1/4）英寸×5英寸×1英寸）对其进行了全面表征。此外，还采用了几种在聚合物上实现智能且功能齐全的芯片实验室的新方法，包括用于自动流体密封的夹管阀、作为采样指示器的旁通通道以及用于延长分析仪使用寿命的坚固连接器设计。使用本研究中开发的功能性聚合物芯片实验室和便携式分析仪，已成功测量了人类全血中的代谢参数，如葡萄糖、乳酸和部分氧气。
