Muthuraman A, Diwan Vishal, Jaggi Amteshwar Singh, Singh Nirmal, Singh Dhandeep
Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India.
J Ethnopharmacol. 2008 Oct 30;120(1):56-62. doi: 10.1016/j.jep.2008.07.049. Epub 2008 Aug 9.
The present study was aimed at investigating the ameliorative effect of Ocimum sanctum in sciatic nerve transection (axotomy)-induced peripheral neuropathy in rats.
Sciatic nerve transection-induced axonal degeneration was assessed histopathologically. Paw pressure, Von Frey Hair, tail cold-hyperalgesia, motor in-coordination tests were performed to assess the extent of neuropathy. Biochemical estimations of thiobarbituric acid reactive species (TBARS), reduced glutathione (GSH), and total calcium levels were also performed. Methanolic extract of Ocimum sanctum at different doses (50, 100 and 200mg/kg p.o.) was administered for 10 consecutive days starting from the day of surgery.
Administration of Ocimum sanctum attenuated sciatic nerve transection-induced axonal degeneration, reduction of nociceptive threshold and motor in-coordination. Moreover, it also attenuated axotomy-induced rise in TBARS, total calcium and decrease in GSH levels in a dose-dependent manner.
Anti-oxidant and calcium attenuating actions may be responsible for observed ameliorative effects of Ocimum sanctum in axotomy-induced neuropathy.
本研究旨在探讨神圣罗勒对大鼠坐骨神经横断（轴突切断）诱导的周围神经病变的改善作用。
通过组织病理学评估坐骨神经横断诱导的轴突变性。进行爪部压力、von Frey毛发、尾部冷痛觉过敏、运动不协调测试以评估神经病变程度。还进行了硫代巴比妥酸反应性物质（TBARS）、还原型谷胱甘肽（GSH）和总钙水平的生化测定。从手术当天开始，连续10天给予不同剂量（50、100和200mg/kg口服）的神圣罗勒甲醇提取物。
给予神圣罗勒可减轻坐骨神经横断诱导的轴突变性、伤害性阈值降低和运动不协调。此外，它还以剂量依赖的方式减轻轴突切断诱导的TBARS、总钙升高以及GSH水平降低。
抗氧化和钙调节作用可能是神圣罗勒在轴突切断诱导的神经病变中观察到的改善作用的原因。
