S100P在体外使卵巢癌细胞对卡铂和紫杉醇敏感。
S100P sensitizes ovarian cancer cells to carboplatin and paclitaxel in vitro.
作者信息
Wang Qi, He Zhijuan, Gao Jianhua, Hu Shuangjiu, Huang Mingli, Liu Min, Zheng Jianhua, Tang Hua
机构信息
The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
出版信息
Cancer Lett. 2008 Dec 18;272(2):277-84. doi: 10.1016/j.canlet.2008.07.017. Epub 2008 Aug 31.
OBJECTIVE
To investigate the relationship between the S100P and the sensitivity of ovarian cancer to chemotherapeutics.
METHOD
We established stable cell lines of ovarian cancer cells, SKOV3 and OVCAR3, that overexpress human S100P. We also transiently transfected the parent cell lines with S100P-targeted siRNA for down-regulation of S100P expression. The sensitivity of all transfected and untransfected cell lines to carboplatin and paclitaxel was detected by MTT assay.
RESULTS
For both cells, IC50s decreased to carboplatin and paclitaxel (p<0.05), with overexpression of S100P compared to untransfected cells. Alternatively, with down-regulation of S100P by siRNA, the IC50 to carboplatin and paclitaxel increased in each case (p<0.05), which was significantly higher compared to untransfected cells.
CONCLUSION
Changes in expression levels of S100P in SKOV3 and OVCAR3 cells results in variable susceptibility to carboplatin and paclitaxel. These data suggest that S100P contributes to chemosensitivity to carboplatin and paclitaxel in ovarian cancer cells.
目的
探讨S100P与卵巢癌对化疗药物敏感性之间的关系。
方法
我们建立了过表达人S100P的卵巢癌细胞稳定细胞系SKOV3和OVCAR3。我们还用靶向S100P的小干扰RNA瞬时转染亲本细胞系，以下调S100P表达。通过MTT法检测所有转染和未转染细胞系对卡铂和紫杉醇的敏感性。
结果
对于这两种细胞，与未转染细胞相比，S100P过表达时，对卡铂和紫杉醇的半数抑制浓度（IC50）降低（p<0.05）。另外，用小干扰RNA下调S后，每种情况下对卡铂和紫杉醇的IC50均增加（p<0.05），与未转染细胞相比显著更高。
结论
SKOV3和OVCAR3细胞中S100P表达水平的变化导致对卡铂和紫杉醇的敏感性不同。这些数据表明，S100P在卵巢癌细胞中对卡铂和紫杉醇的化疗敏感性有影响。 100P
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