Wang Xingzu, Cheng Xiang, Sun Dezhi
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, People's Republic of China.
Appl Microbiol Biotechnol. 2008 Oct;80(5):907-15. doi: 10.1007/s00253-008-1657-1. Epub 2008 Sep 2.
Autocatalysis in biological decolorization of Reactive Black 5 (RB5) by Rhodopseudomonas palustris W1 was investigated in batch assays. An improvement of 1.5-fold in decolorization rate of RB5 was obtained by supplementing decolorization metabolites from 200 mg l(-1) RB5. Liquid chromatography-mass spectrometry and cyclic voltammetric analysis revealed that the constituent of dye precursors, from azo bonds breakage, with quinone-like structure and reversible oxidation-reduction activity can be used as redox mediators and was responsible for the catalytic reduction of RB5. The required amount of metabolites for catalytic decolorization was quite small, indicating its possible application in real textile wastewater treatment. Furthermore, decolorization metabolites of RB5 were shown as effective in catalyzing anaerobic decolorization of Direct Yellow 11, an azo dye without autocatalyic activity.
通过批次试验研究了沼泽红假单胞菌W1对活性黑5（RB5）进行生物脱色过程中的自催化作用。通过添加200 mg l(-1) RB5脱色代谢产物，RB5脱色率提高了1.5倍。液相色谱 - 质谱联用和循环伏安分析表明，偶氮键断裂产生的具有醌类结构和可逆氧化还原活性的染料前体成分可作为氧化还原介质，负责催化还原RB5。催化脱色所需的代谢产物量非常少，表明其在实际纺织废水处理中的应用潜力。此外，RB5的脱色代谢产物对直接黄11的厌氧脱色具有催化作用，直接黄11是一种无自催化活性的偶氮染料。
