Bandyopadhyay Samiran, Sasmal Debasis, Dutta Tapan Kumar, Ghosh Monoj Kumar, Sarkar Mihir, Sasmal Nihar Kanta, Bhattacharya Mohan
National Research Centre on Yak, Dirang, Arunachal Pradesh 790 101, India.
Trop Anim Health Prod. 2009 Apr;41(4):587-92. doi: 10.1007/s11250-008-9228-0. Epub 2008 Sep 2.
The present study was carried out to explore the seroprevalence of brucellosis in yaks of North-Eastern hilly yak tracts of Arunachal Pradesh, India. Of 374 animals tested, 23.79, 21.11 and 18.98% were found positive for brucellosis using avidin-biotin ELISA (AB-ELISA), Rose-Bengal plate test (RBPT) and standard tube-agglutination test (STAT), respectively. The relative sensitivity and specificity for STAT were 79.77 and 100%, respectively and the same for RBPT were 88.76 and 100%, respectively in comparison to AB-ELISA. The alarming prevalence as recorded was highest among the yak cows (31.42%) followed by heifers (23.85%) and bulls (8.88%). The immune response in yaks following standard dose of calfhood vaccination with Brucella abortus strain 19 vaccine showed that protective antibody level persisted up to 210 days. This is the first report from India on prevalence of brucellosis and immunization with B abortus strain 19 vaccine in yaks. The present investigation would be a valuable guideline for future control measure and eradication programme of brucellosis in yaks.
本研究旨在探究印度阿鲁纳恰尔邦东北部丘陵牦牛产区牦牛布鲁氏菌病的血清流行率。在检测的374头动物中，使用抗生物素蛋白-生物素酶联免疫吸附测定法（AB-ELISA）、玫瑰红平板试验（RBPT）和标准试管凝集试验（STAT）检测布鲁氏菌病，阳性率分别为23.79%、21.11%和18.98%。与AB-ELISA相比，STAT的相对敏感性和特异性分别为79.77%和100%，RBPT的相对敏感性和特异性分别为88.76%和100%。记录到的惊人流行率在牦牛母牛中最高（31.42%），其次是小母牛（23.85%）和公牛（8.88%）。用流产布鲁氏菌19疫苗对牦牛进行标准剂量的幼年接种后，牦牛的免疫反应表明保护性抗体水平可持续210天。这是印度关于牦牛布鲁氏菌病流行率和用流产布鲁氏菌19疫苗免疫的首份报告。本调查将为未来牦牛布鲁氏菌病的控制措施和根除计划提供有价值的指导。
