Gao Chang-Ming, Takezaki Toshiro, Wu Jian-Zhong, Zhang Xiao-Mei, Cao Hai-Xia, Ding Jian-Hua, Liu Yan-Ting, Li Su-Ping, Cao Jia, Matsuo Keitaro, Hamajima Nobuyuki, Tajima Kazuo
Division of Epidemiology, Jiangsu Provincial Institute of Cancer Research, 42 Baiziting, Nanjing, China.
World J Gastroenterol. 2008 Aug 28;14(32):5078-83. doi: 10.3748/wjg.14.5078.
To evaluate the relationship between drinking and polymorphisms of alcohol dehydrogenase 2 (ADH2) and/or aldehyde dehydrogenase 2 (ALDH2) for risk of colorectal cancer (CRC) in Chinese males.
A case-control study was conducted in 190 cases and 223 population-based controls. ADH2 Arg47His (G-A) and ALDH2 Glu487Lys (G-A) genotypes were identified by PCR and denaturing high-performance liquid chromatography (DHPLC). Information on smoking and drinking was collected and odds ratio (OR) was estimated.
The ADH2 A/A and ALDH2 G/G genotypes showed moderately increased CRC risk. The age- and smoking-adjusted OR for ADH2 A/A relative to G/A and G/G was 1.60 (95% CI=1.08-2.36), and the adjusted OR for ALDH2 G/G relative to G/A and A/A was 1.79 (95% CI=1.19-2.69). Significant interactions between ADH2, ALDH2 and drinking were observed. As compared to the subjects with ADH2 G and ALDH2 A alleles, those with ADH2 A/A and ALDH2 G/G genotypes had a significantly increased OR (3.05, 95% CI= 1.67-5.57). The OR for CRC among drinkers with the ADH2 A/A genotype was increased to 3.44 (95% CI= 1.84-6.42) compared with non-drinkers with the ADH2 G allele. The OR for CRC among drinkers with the ALDH2 G/G genotype was also increased to 2.70 (95% CI= 1.57-4.66) compared with non-drinkers with the ALDH2 A allele.
Polymorphisms of the ADH2 and ALDH2 genes are significantly associated with CRC risk. There are also significant gene-gene and gene-environment interactions between drinking and ADH2 and ALDH2 polymorphisms regarding CRC risk in Chinese males.
评估饮酒与乙醇脱氢酶2（ADH2）和/或乙醛脱氢酶2（ALDH2）基因多态性在中国男性结直肠癌（CRC）风险中的关系。
对190例病例和223例基于人群的对照进行病例对照研究。通过聚合酶链反应（PCR）和变性高效液相色谱（DHPLC）鉴定ADH2 Arg47His（G-A）和ALDH2 Glu487Lys（G-A）基因型。收集吸烟和饮酒信息并估计比值比（OR）。
ADH2 A/A和ALDH2 G/G基因型显示CRC风险适度增加。ADH2 A/A相对于G/A和G/G的年龄和吸烟调整后OR为1.60（95%可信区间[CI]=1.08-2.36），ALDH2 G/G相对于G/A和A/A的调整后OR为1.79（95%CI=1.19-2.69）。观察到ADH2、ALDH2与饮酒之间存在显著的交互作用。与携带ADH2 G和ALDH2 A等位基因的受试者相比，携带ADH2 A/A和ALDH2 G/G基因型的受试者OR显著增加（3.05，95%CI=1.67-5.57）。与携带ADH2 G等位基因的非饮酒者相比，携带ADH2 A/A基因型的饮酒者患CRC的OR增加到3.44（95%CI=1.84-6.42）。与携带ALDH2 A等位基因的非饮酒者相比，携带ALDH2 G/G基因型的饮酒者患CRC的OR也增加到2.70（95%CI=1.57-4.66）。
ADH2和ALDH2基因多态性与CRC风险显著相关。在中国男性中，饮酒与ADH2和ALDH2基因多态性在CRC风险方面也存在显著的基因-基因和基因-环境交互作用。
