Zhao Zhan-yu, Wu Tian-xing, Tang Hong-gang, Zhang Ji-ze
Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
J Zhejiang Univ Sci B. 2008 Sep;9(9):691-700. doi: 10.1631/jzus.B0820181.
We examined the effects of conjugated linoleic acid (CLA) on growth, fatty acid composition and enzyme activity of fatty acid oxidation in the liver of large yellow croaker. We divided 1600 fish (average initial weight 150 g) into 4 groups and reared them in 8 cages. Four dietary treatments were formulated to contain 0%, 1%, 2% and 4% (w/w) CLA, respectively. The fish were fed for 10 weeks ad libitum twice daily. We found that the dietary CLA had no effect on growth, biometric parameters and whole body proximate (P>0.05), but showed some significant effects on the fatty acid composition in both muscle and the liver. The activities of lipogenic enzymes were slightly depressed in fish fed with increasing levels of CLA when compared with control (P>0.05). Dietary CLA supplementation had no effects on liver lipid content, but significantly increased the contents of saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA) (P<0.05) and decreased monounsaturated fatty acid (MUFA) content in both muscle and the liver. Dietary CLA inclusion resulted in significant increases of the biologically active cis-9, trans-11 and trans-10, cis-12 isomers in both tissues (P<0.05). The total accumulation of CLA was higher in the liver (3.83%, w/w) than in muscle (3.77%, w/w) when fed with 4% (w/w) CLA. This study demonstrates that large yellow croakers are capable of absorbing and depositing CLA and long-chain n-3 PUFA in the liver and muscle, showing that this species fed with CLA could be an important human food source for these healthful fatty acids.
我们研究了共轭亚油酸（CLA）对大黄鱼生长、脂肪酸组成及肝脏脂肪酸氧化酶活性的影响。我们将1600尾鱼（平均初始体重150克）分为4组，饲养在8个网箱中。配制了四种日粮处理，分别含有0%、1%、2%和4%（w/w）的CLA。鱼每天投喂两次，随意采食，持续10周。我们发现，日粮CLA对生长、生物测量参数和鱼体常规成分没有影响（P>0.05），但对肌肉和肝脏中的脂肪酸组成有一些显著影响。与对照组相比，随着CLA水平的增加，投喂CLA的鱼中脂肪生成酶的活性略有降低（P>0.05）。日粮添加CLA对肝脏脂质含量没有影响，但显著增加了肌肉和肝脏中饱和脂肪酸（SFA）和多不饱和脂肪酸（PUFA）的含量（P<0.05），并降低了单不饱和脂肪酸（MUFA）的含量。日粮中添加CLA导致两个组织中生物活性顺-9，反-11和反-10，顺-12异构体显著增加（P<0.05）。当投喂4%（w/w）CLA时，肝脏中CLA的总积累量（3.83%，w/w）高于肌肉（3.77%，w/w）。本研究表明，大黄鱼能够在肝脏和肌肉中吸收和沉积CLA以及长链n-3多不饱和脂肪酸，表明投喂CLA的该物种可能是这些有益脂肪酸的重要人类食物来源。
