海洋胶原蛋白肽对延缓皮肤衰老的作用

[Effects of marine collagen peptide on delaying the skin aging].

作者信息

Pei Xin-rong, Yang Rui-yue, Zhang Zhao-feng, Xu Ya-jun, Han Xiao-long, Wang Jun-bo, Li Yong

机构信息

Department of Food and Nutritional Hygiene, School of Public Health, Beijing University, Beijing 100083, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2008 Apr;42(4):235-8.

PMID:18763632

Abstract

OBJECTIVE

To investigate the protective effect of marine collagen peptides (MCPs) on the skin of aged mice induced by D-galactose.

METHODS

Subchronic toxicity study was conducted while D-galactose induced subacute aging model was established. D-galactose dose of 0.125 g/kg body weight was intraperitoneally injected daily for 90 days. Marine collagen peptide 0.225, 0.450, 1.350 g/kg body weight were administered by oral gavage. Superoxide dismutase (SOD), catalase (CAT) activity and malondialdehyde (MDA) content in blood serum were measured, along with cutaneous histopathology examination.

RESULTS

Epidermal thickness was significantly higher in MCPs treated group. Number and activity of fibroblast in MCPs treated dermis was increased prominently. The activity of SOD in 0.225, 0.450, 1.350 g/kgbw MCPs treated groups were 455.52 +/- 11.39, 460.15 +/- 18.09, 468.59 +/- 27.25 U/ml respectively, each of which was significantly higher than that in model control group; the activity of serum CAT in 0.225, 1.350 g/kgbw MCPs treated groups (21.33 +/- 4.82, 21.69 +/- 1.68 U/ml) were obviously increased compared with that in model control group (17.14 +/- 2.81 U/ml); MDA level in 0.450, 1.350 g/kgbw MCPs treated groups were 5.67 +/- 0.93, 5.76 +/- 1.02 nmol/ml respectively, each of which was significantly lower than that in model control group (7.63 +/- 1.37 nmol/ml).

CONCLUSIONS

The results showed that MCPs might play a protective role on skin aging by improving the activity of antioxidant.

摘要

目的

研究海洋胶原蛋白肽（MCPs）对D-半乳糖诱导的衰老小鼠皮肤的保护作用。

方法

在建立D-半乳糖诱导的亚急性衰老模型的同时进行亚慢性毒性研究。每天腹腔注射剂量为0.125 g/kg体重的D-半乳糖，持续90天。通过口服灌胃给予体重为0.225、0.450、1.350 g/kg的海洋胶原蛋白肽。测定血清中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性和丙二醛（MDA）含量，并进行皮肤组织病理学检查。

结果

MCPs处理组的表皮厚度显著更高。MCPs处理的真皮中成纤维细胞的数量和活性显著增加。0.225、0.450、1.350 g/kgbw MCPs处理组的SOD活性分别为455.52±11.39、460.15±18.09、468.59±27.25 U/ml，均显著高于模型对照组；0.225、1.350 g/kgbw MCPs处理组的血清CAT活性（21.33±4.82、21.69±1.68 U/ml）与模型对照组（17.14±2.81 U/ml）相比明显升高；0.450、1.350 g/kgbw MCPs处理组的MDA水平分别为5.67±0.93、5.76±1.02 nmol/ml，均显著低于模型对照组（7.63±1.37 nmol/ml）。