二甲基乙醇胺和复合氨基酸对D-半乳糖诱导的大鼠皮肤衰老模型的影响

Effects of dimethylaminoethanol and compound amino acid on D-galactose induced skin aging model of rat.

作者信息

Liu Su, Chen Zhenyu, Cai Xia, Sun Ying, Zhao Cailing, Liu Fangjun, Liu Dalie

机构信息

Department of Plastic Surgery, Zhujiang Hospital, Southern Medical University, 253 GongYe Middle Avenue, Guangzhou, Guangdong 510280, China ; Department of Plastic and Aesthetic Surgery, The Affiliated Hospital of Medical School, Qingdao University, Qingdao, Shandong 266003, China.

Department of Plastic and Aesthetic Surgery, The Affiliated Hospital of Medical School, Qingdao University, Qingdao, Shandong 266003, China.

出版信息

ScientificWorldJournal. 2014;2014:507351. doi: 10.1155/2014/507351. Epub 2014 Jul 14.

DOI:10.1155/2014/507351

PMID:25133239

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4123624/

Abstract

A lasting dream of human beings is to reverse or postpone aging. In this study, dimethylaminoethanol (DMAE) and compound amino acid (AA) in Mesotherapy were investigated for their potential antiaging effects on D-galactose induced aging skin. At 18 days after D-gal induction, each rat was treated with intradermal microinjection of saline, AA, 0.1% DMAE, 0.2% DMAE, 0.1% DMAE + AA, or 0.2% DMAE + AA, respectively. At 42 days after treatment, the skin wound was harvested and assayed. Measurement of epidermal and dermal thickness in 0.1% DMAE + AA and 0.2% DMAE + AA groups appeared significantly thicker than aging control rats. No differences were found in tissue water content among groups. Hydroxyproline in 0.1% DMAE + AA, 0.2% DMAE + AA, and sham control groups was much higher than all other groups. Collagen type I, type III, and MMP-1 expression was highly upregulated in both 0.1% DMAE + AA and 0.2% DMAE + AA groups compared with aging control. In contrast, TIMP-1 expression levels of various aging groups were significantly reduced when compared to sham control. Coinjection of DMAE and AA into target tissue has marked antiaging effects on D-galactose induced skin aging model of rat.

摘要

人类长久以来的梦想是逆转或延缓衰老。在本研究中，对中胚层疗法中的二甲基乙醇胺（DMAE）和复合氨基酸（AA）在D-半乳糖诱导的衰老皮肤中的潜在抗衰作用进行了研究。在D-半乳糖诱导18天后，分别对每只大鼠进行皮内微量注射生理盐水、AA、0.1% DMAE、0.2% DMAE、0.1% DMAE + AA或0.2% DMAE + AA处理。处理42天后，采集皮肤伤口并进行检测。0.1% DMAE + AA组和0.2% DMAE + AA组的表皮和真皮厚度测量结果显示明显厚于衰老对照大鼠。各组间组织含水量未发现差异。0.1% DMAE + AA组、0.2% DMAE + AA组和假手术对照组的羟脯氨酸含量远高于其他所有组。与衰老对照组相比，0.1% DMAE + AA组和0.2% DMAE + AA组的I型、III型胶原蛋白和MMP-1表达均显著上调。相反，与假手术对照组相比，各衰老组的TIMP-1表达水平均显著降低。将DMAE和AA共同注射到靶组织中对D-半乳糖诱导的大鼠皮肤衰老模型具有显著的抗衰作用。