Wang Junbo, Zhang Zhaofeng, Pei Xinrong, Yang Ruiyue, Li Yong
Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China.
Wei Sheng Yan Jiu. 2010 Mar;39(2):143-6.
To observe the effects of marine collagen peptides (MCPs) on blood glucose and lipid metabolism in hyperinsulinemic rats.
Male SD rats fed with high fat diet for 4 months were used to establish a hyperinsulinemic model. Thirty two hyperinsulinemic rats were divided into four groups according to body weight and serum insulin level, namely hyperinsulinemic control (HIC) group and 3 Marine Collagen Peptides treated groups with 8 rats in each group. Another 8 healthy male SD rats fed with basic diet were used as negative control (NC). Rats in MCPs groups were treated by intragastric way with MCPs at the dose of 0.225 g/kg bw, 0.45 g/kg bw and 1.35 g/kg bw respectively for 8 weeks, while rats in HIC and NC groups were treated with tap water in the same manner. During the test period, rats in HIC and MCPs groups were fed with high fat diet sequentially, while rats in NC group were fed with basic diet. Fasting serum glucose (GLU), insulin (INS) and lipids were assayed respectively before and after MCPs treatment. Oral glucose tolerance test, antioxidase activity, serum malondialdehyde (MDA) levels and the ultrastructure of islet beta cell were carried out at the end of the experiment.
After 8 weeks of treatment, compared with HIC group, fasting INS, TC and TG of all three MCPs-treated groups decreased obviously, while fasting GLU level lowered significantly in 0.225 g/kg bw and 1.35 g/kg bw MCPs groups. Moreover, GLU level at 0.5 h, 2 h and the area under blood sugar curve in OGTT were evidently lower in 0.45 g/kg bw and 1.35 g/kg bw MCPs groups. Besides, serum SOD activity increased in all MCPs groups, and serum GSH-Px activity elevated obviously in 1.35 g/kg bw MCPs group, while serum MDA reduced. Finally, the ultrastructural impairment of islet resulted from high fat diet was improved evidently by 1.35 g/kg bw MCPs.
MCPs was effective in improving glucose and lipid metabolism in hyperinsulinemic rats induced by high fat diet.
观察海洋胶原蛋白肽（MCPs）对高胰岛素血症大鼠血糖和脂质代谢的影响。
选取雄性SD大鼠，高脂饮食喂养4个月以建立高胰岛素血症模型。32只高胰岛素血症大鼠根据体重和血清胰岛素水平分为四组，即高胰岛素血症对照组（HIC）和3个海洋胶原蛋白肽治疗组，每组8只。另取8只喂饲基础饲料的健康雄性SD大鼠作为阴性对照组（NC）。MCPs组大鼠分别以0.225 g/kg体重、0.45 g/kg体重和1.35 g/kg体重的剂量灌胃给予MCPs，持续8周，而HIC组和NC组大鼠以同样方式给予自来水。在试验期间，HIC组和MCPs组大鼠依次喂饲高脂饲料，而NC组大鼠喂饲基础饲料。分别在MCPs治疗前后测定空腹血清葡萄糖（GLU）、胰岛素（INS）和脂质水平。在实验结束时进行口服葡萄糖耐量试验、抗氧化酶活性、血清丙二醛（MDA）水平及胰岛β细胞超微结构检测。
治疗8周后，与HIC组相比，三个MCPs治疗组的空腹INS、TC和TG均明显降低，而0.225 g/kg体重和1.35 g/kg体重MCPs组的空腹GLU水平显著降低。此外，0.45 g/kg体重和1.35 g/kg体重MCPs组在OGTT中0.5 h、2 h时的GLU水平及血糖曲线下面积明显较低。此外，所有MCPs组血清SOD活性均升高，1.35 g/kg体重MCPs组血清GSH-Px活性明显升高，而血清MDA降低。最后，1.35 g/kg体重MCPs明显改善了高脂饮食所致的胰岛超微结构损伤。
MCPs对高脂饮食诱导的高胰岛素血症大鼠的血糖和脂质代谢具有改善作用。
