Li Minyong, Lin Na, Huang Zhen, Du Lupei, Altier Craig, Fang Hao, Wang Binghe
Department of Chemistry and Center for Biotechnology and Drug Design, Georgia State University, Atlanta, Georgia 30302-4098, USA.
J Am Chem Soc. 2008 Sep 24;130(38):12636-8. doi: 10.1021/ja801510d. Epub 2008 Sep 3.
The first general method for the selection of boronic acid-based aptamers (boronolectins) that allows for glycan substructure focusing is described. Using fibrinogen as a model glycoprotein, we have selected boronic acid-modified DNA aptamers that have high affinities (low nM Kd) and the ability to recognize changes in the glycosylation site. The method developed should also be applicable to the development of aptamers for other glycoproducts, such as glycolipids and glycopeptides.
本文描述了第一种用于选择基于硼酸的适配体(硼凝集素)的通用方法,该方法能够聚焦聚糖亚结构。以纤维蛋白原为模型糖蛋白,我们筛选出了具有高亲和力(低纳摩尔解离常数)且能够识别糖基化位点变化的硼酸修饰的DNA适配体。所开发的方法也应适用于其他糖产物(如糖脂和糖肽)适配体的开发。