Futane Abhishek, Narayanamurthy Vigneswaran, Jadhav Pramod, Srinivasan Arthi
Fakulti Kejuruteraan Elektronik Dan Kejuruteraan Komputer, Universiti Teknikal Malaysia Melaka, Hang Tuah Jaya, Durian Tunggal, 76100 Melaka, Malaysia.
Advance Sensors and Embedded Systems (ASECs), Centre for Telecommunication Research and Innovation, Fakulti Teknologi Kejuruteraan Elektrik Dan Elektronik, Universiti Teknikal Malaysia Melaka, Hang Tuah Jaya, Durian Tunggal, 76100 Melaka, Malaysia.
Microfluid Nanofluidics. 2023;27(2):15. doi: 10.1007/s10404-022-02622-3. Epub 2023 Jan 18.
Aptasensors have attracted considerable interest and widespread application in point-of-care testing worldwide. One of the biggest challenges of a point-of-care (POC) is the reduction of treatment time compared to central facilities that diagnose and monitor the applications. Over the past decades, biosensors have been introduced that offer more reliable, cost-effective, and accurate detection methods. Aptamer-based biosensors have unprecedented advantages over biosensors that use natural receptors such as antibodies and enzymes. In the current epidemic, point-of-care testing (POCT) is advantageous because it is easy to use, more accessible, faster to detect, and has high accuracy and sensitivity, reducing the burden of testing on healthcare systems. POCT is beneficial for daily epidemic control as well as early detection and treatment. This review provides detailed information on the various design strategies and virus detection methods using aptamer-based sensors. In addition, we discussed the importance of different aptamers and their detection principles. Aptasensors with higher sensitivity, specificity, and flexibility are critically discussed to establish simple, cost-effective, and rapid detection methods. POC-based aptasensors' diagnostic applications are classified and summarised based on infectious and infectious diseases. Finally, the design factors to be considered are outlined to meet the future of rapid POC-based sensors.
适体传感器在全球即时检测中引起了广泛关注并得到了广泛应用。即时检测（POC）面临的最大挑战之一是与进行诊断和监测应用的中心设施相比，缩短治疗时间。在过去几十年中，已经引入了生物传感器，它们提供了更可靠、更具成本效益和更准确的检测方法。基于适体的生物传感器相对于使用抗体和酶等天然受体的生物传感器具有前所未有的优势。在当前的疫情中，即时检测（POCT）具有优势，因为它易于使用、更易获取、检测速度更快，并且具有高准确性和灵敏度，减轻了医疗系统的检测负担。POCT对日常疫情防控以及早期检测和治疗都有益处。本综述提供了有关使用基于适体的传感器的各种设计策略和病毒检测方法的详细信息。此外，我们讨论了不同适体的重要性及其检测原理。对具有更高灵敏度、特异性和灵活性的适体传感器进行了批判性讨论，以建立简单、经济高效且快速的检测方法。基于POC的适体传感器的诊断应用根据传染病和非传染病进行了分类和总结。最后，概述了为满足基于POC的快速传感器的未来发展而需考虑的设计因素。
