McClenahan Shasta D, Burek Kathy A, Beckmen Kimberlee B, Knowles Nick J, Neill John D, Romero Carlos H
Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, 2015 SW 16th Avenue, Building 1017, Gainesville, FL 32610, USA.
Virus Res. 2008 Dec;138(1-2):26-35. doi: 10.1016/j.virusres.2008.08.002. Epub 2008 Sep 16.
Marine vesiviruses were isolated in cell culture from oral and rectal swabs and vesicular fluid from Alaskan Steller sea lions (SSL; Eumetopias jubatus). Further characterization by RT-PCR, complete genomic sequencing, and phylogenetic analyses indicated that these viruses are most closely related to the marine vesiviruses, but are distinct viruses and represent two novel genotypes. The complete genome of these two SSL isolates was sequenced after cloning their viral cDNA. The genomes were found to be 8302 and 8305 nucleotides in length, organized in three open reading frames and contained 5' and 3' untranslated regions (UTR) of 19 and 180 nucleotides, respectively. The complete genomes of both SSL viruses were most closely related to each other and shared 83.0% nucleotide identity. Using the very limited number of complete genomic vesivirus sequences available in the NCBI database, these novel SSL vesiviruses seem most closely related to vesicular exanthema of swine virus-A48 and least related to rabbit vesivirus and walrus calicivirus. Specific antiserum against some evolutionary closer marine vesiviruses did not neutralize these isolates supporting the novel nature of these SSL viruses.
从阿拉斯加北海狗（Eumetopias jubatus）的口腔和直肠拭子以及水疱液中，通过细胞培养分离出了海洋杯状病毒。通过逆转录聚合酶链反应（RT-PCR）、全基因组测序和系统发育分析进一步鉴定表明，这些病毒与海洋杯状病毒关系最为密切，但却是不同的病毒，代表了两种新的基因型。在克隆这两种北海狗分离株的病毒互补脱氧核糖核酸（cDNA）后，对其全基因组进行了测序。发现这些基因组长度分别为8302和8305个核苷酸，由三个开放阅读框组成，并分别包含19个和180个核苷酸的5'和3'非翻译区（UTR）。这两种北海狗病毒的全基因组彼此关系最为密切，核苷酸同一性为83.0%。利用美国国立医学图书馆（NCBI）数据库中非常有限的全基因组杯状病毒序列，这些新的北海狗杯状病毒似乎与猪水疱性疹病毒-A48关系最为密切，与兔杯状病毒和海象杯状病毒关系最不密切。针对一些进化关系较近的海洋杯状病毒的特异性抗血清不能中和这些分离株，这支持了这些北海狗病毒的新颖性。
