通过对病毒DNA进行限制性内切酶分析鉴别减蛋综合征病毒分离株

Differentiation of egg drop syndrome virus isolates by restriction endonuclease analysis of virus DNA.

作者信息

Todd D, McNulty M S, Smyth J A

机构信息

Veterinary Research Laboratories, Stormont, Belfast, Northern Ireland.

出版信息

Avian Pathol. 1988;17(4):909-19. doi: 10.1080/03079458808436512.

DOI:10.1080/03079458808436512

PMID:18766751

Abstract

Thirteen isolates of egg drop syndrome (EDS) virus were compared by restriction endonuclease analysis of the virus DNA. One virus, an Australian chicken isolate, was distinguished from the others using the endonucleases EcoRl, BamUl, Kpnl, Hindlll, Pstl and Pvull, all of which recognise six base pair DNA sequences. Polyacrylamide gel restriction fragment patterns generated by Haelll, Hhall and TaqI, which recognise four base pairs, allowed further differentiation of the virus isolates. Nine chicken viruses isolated in the United Kingdom and Belgium in the period 1976 to 1987 were identical and could be distinguished from three United Kingdom duck isolates. The Australian isolate, in addition to possessing a DNA deletion (0.4 kbp) at one end of the genome (32.6 kbp) also differed from the European isolates at base sequence level. Genome maps for EcoRl, BamHl, Kpnl and Pstl are reported for the 127 isolate of EDS virus.

摘要

通过对病毒DNA进行限制性内切酶分析，对13株减蛋综合征（EDS）病毒进行了比较。使用识别六碱基对DNA序列的内切酶EcoRl、BamUl、Kpnl、Hindlll、Pstl和Pvull，将一株澳大利亚鸡源病毒与其他病毒区分开来。识别四碱基对的HaeIII、HhaI和TaqI产生的聚丙烯酰胺凝胶限制性片段图谱，使病毒分离株能够进一步区分。1976年至1987年期间在英国和比利时分离出的9株鸡病毒是相同的，并且可以与3株英国鸭源分离株区分开来。澳大利亚分离株除了在基因组（32.6 kbp）一端有一个DNA缺失（0.4 kbp）外，在碱基序列水平上也与欧洲分离株不同。报告了EDS病毒127分离株的EcoRl、BamHl、Kpnl和Pstl的基因组图谱。