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一些欧美禽传染性喉气管炎病毒分离株的限制性内切酶图谱

Restriction endonuclease patterns of some European and American isolates of avian infectious laryngotracheitis virus.

作者信息

Leib D A, Bradbury J M, Gaskell R M, Hughes C S, Jones R C

出版信息

Avian Dis. 1986 Oct-Dec;30(4):835-7.

PMID:3028360
Abstract

Eleven isolates of infectious laryngotracheitis virus (nine European and two American) were compared by restriction endonuclease analysis of their DNA after radiolabeling with 32P. Digestion with KpnI gave identical cleavage patterns for all the European isolates, but the two American viruses (one field and one vaccine) showed some differences from them and from each other. In the case of the American vaccine strain, however, these differences were only minor. After BamHI digestion, only the American field isolate appeared to be different, whereas with HindIII, all 11 isolates were identical.

摘要

通过用32P进行放射性标记后对其DNA进行限制性内切酶分析,比较了11株传染性喉气管炎病毒分离株(9株欧洲分离株和2株美国分离株)。用KpnI消化后,所有欧洲分离株产生相同的切割模式,但2株美国病毒(1株野毒株和1株疫苗株)与欧洲分离株以及它们彼此之间存在一些差异。然而,对于美国疫苗株,这些差异较小。用BamHI消化后,只有美国野毒株出现差异,而用HindIII消化后,所有11株分离株都相同。

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