Martel An, Pasmans Frank, Hellebuyck Tom, Haesebrouck Freddy, Vandamme Peter
Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium.
Int J Syst Evol Microbiol. 2008 Sep;58(Pt 9):2206-9. doi: 10.1099/ijs.0.65478-0.
Five bacterial isolates were recovered from dermatitis or organ lesions of five agamid lizards. Three strains were recovered from Uromastyx species with dermatitis or septicaemia. All five isolates were Gram-positive small rods that could not be identified using conventional phenotypic systems. They grew on sheep blood agar as small haemolytic colonies after 24 h of incubation at temperatures between 25 and 42 degrees C under aerobic, microaerophilic or anaerobic conditions. They were catalase-positive and non-motile. Comparative analysis of 16S rRNA gene sequences revealed that the strains represent a new taxon within the class Actinobacteria. Their nearest phylogenetic neighbours were determined as Brachybacterium faecium LMG 19847(T) (95.9% 16S rRNA gene sequence similarity) and Dermabacter hominis NCIMB 13131(T) (95.3% similarity). The DNA G+C content of one of the novel isolates, strain IMP2(T), was 61 mol%. On the basis of morphological, chemotaxonomic and phylogenetic differences from other species of coryneform bacteria, it is proposed that this novel taxon be classified as Devriesea agamarum gen. nov., sp. nov. The type strain is IMP2(T) (=LMG 24257(T)=CCUG 55056(T)).
从5只鬣蜥的皮炎或器官损伤中分离出5株细菌。3株菌株从患有皮炎或败血症的刺尾蜥属物种中分离得到。所有5株分离菌均为革兰氏阳性小杆菌，无法用传统表型系统鉴定。在25至42摄氏度的温度下，于需氧、微需氧或厌氧条件下培养24小时后，它们在绵羊血琼脂上形成小的溶血菌落。它们过氧化氢酶阳性且不运动。16S rRNA基因序列的比较分析表明，这些菌株代表放线菌纲内的一个新分类单元。确定其最接近的系统发育邻居为粪短杆菌LMG 19847(T)（16S rRNA基因序列相似性为95.9%）和人皮杆菌NCIMB 13131(T)（相似性为95.3%）。其中一株新分离菌IMP2(T)的DNA G+C含量为61 mol%。基于与其他棒状杆菌属物种在形态、化学分类和系统发育上的差异，建议将这个新分类单元归类为阿加马德弗里斯菌属，新属，新种。模式菌株为IMP2(T)（=LMG 24257(T)=CCUG 55056(T)）。
