Dastager Syed G, Lee Jae-Chan, Ju Yoon-Jung, Park Dong-Jin, Kim Chang-Jin
Functional Metabolomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
Syst Appl Microbiol. 2008 Mar;31(1):24-9. doi: 10.1016/j.syapm.2007.10.003. Epub 2008 Jan 8.
A strictly aerobic, Gram-positive, motile, coccoid-shaped, halotolerant actinobacterium (10% NaCl, w/v), designated MSL-23T, was isolated from a soil sample on Bigeum Island, Korea. Results of 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Nocardioides, with the highest sequence similarity (95.63%) being to Nocardioides kribbensis KCTC 19038T. The major menaquinone was MK-8(H4), and the predominant cellular fatty acids were i-C16:0, ai-C17:0, C18:1 omega 9c and 10-methyl-C16:0. The DNA G+C content was 69.7 mol%. The 16S rRNA gene sequence of strain MSL 23T and its chemotaxonomic properties showed it to be unique in the genus Nocardioides. Phenotypic characteristics distinguished strain MSL-23T from other Nocardioides species. On the basis of the phenotypic, chemotaxonomic and phylogenetic data strain MSL-23T represents a novel species, for which the name Nocardioides halotolerans sp. nov. is proposed, with MSL-23T (=KCTC 19274T=DSM 19273T) as the type strain.
从韩国比格姆岛的一份土壤样本中分离出一株严格需氧、革兰氏阳性、具运动性、球状、耐盐的放线菌（10% NaCl，w/v），命名为MSL-23T。16S rRNA基因序列分析结果表明，该分离株属于诺卡氏菌属，与Kribbensis诺卡氏菌KCTC 19038T的序列相似性最高（95.63%）。主要甲基萘醌为MK-8(H4)，主要细胞脂肪酸为i-C16:0、ai-C17:0、C18:1 ω 9c和10-甲基-C16:0。DNA G+C含量为69.7 mol%。菌株MSL 23T的16S rRNA基因序列及其化学分类学特性表明它在诺卡氏菌属中是独特的。表型特征将菌株MSL-23T与其他诺卡氏菌属物种区分开来。基于表型、化学分类学和系统发育数据，菌株MSL-23T代表一个新物种，为此提出名为耐盐诺卡氏菌（Nocardioides halotolerans sp. nov.）的名称，以MSL-23T（=KCTC 19274T=DSM 19273T）作为模式菌株。
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