Kim Hye Min, Choi Dong Han, Hwang Chung Yeon, Cho Byung Cheol
School of Earth and Environmental Sciences, Seoul National University, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Sep;58(Pt 9):2056-64. doi: 10.1099/ijs.0.65569-0.
A rod-shaped marine bacterium, designated CL-Z59(T), was isolated from seawater enriched with zooplankton. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CL-Z59(T) belongs to the genus Nocardioides, the highest level of sequence similarity (99.2%) being found with respect to Nocardioides marinisabuli KCCM 42681(T). However, DNA-DNA hybridization experiments between strain CL-Z59(T) and N. marinisabuli KCCM 42681(T) revealed a mean relatedness value of 33%. Cells were Gram-positive, strictly aerobic and non-motile. Strain CL-Z59(T) grew optimally at pH 6-7 and 25-30 degrees C and at a NaCl concentration of 3%. The isolate was characterized chemotaxonomically as having LL-diaminopimelic acid in the cell wall. The major isoprenoid quinone was MK-8(H(4)) and the predominant cellular fatty acid was iso-C(16:0). The DNA G+C content was 73.3 mol%. On the basis of phylogenetic, genotypic and phenotypic data, strain CL-Z59(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides salarius sp. nov. is proposed. The type strain is CL-Z59(T) (=KCCM 42320(T)=DSM 18239(T)).
从富含浮游动物的海水中分离出一株杆状海洋细菌，命名为CL-Z59(T)。基于16S rRNA基因序列的系统发育分析表明，菌株CL-Z59(T)属于诺卡氏菌属，与海沙诺卡氏菌KCCM 42681(T)的序列相似性最高（99.2%）。然而，菌株CL-Z59(T)与海沙诺卡氏菌KCCM 42681(T)之间的DNA-DNA杂交实验显示平均相关性值为33%。细胞为革兰氏阳性，严格需氧且不运动。菌株CL-Z59(T)在pH 6-7、25-30℃和3%的NaCl浓度下生长最佳。该分离株经化学分类学鉴定，细胞壁含有LL-二氨基庚二酸。主要类异戊二烯醌为MK-8(H4)，主要细胞脂肪酸为异-C16:0。DNA G+C含量为73.3 mol%。基于系统发育、基因型和表型数据，菌株CL-Z59(T)代表诺卡氏菌属的一个新物种，为此提出新名称海沙诺卡氏菌（Nocardioides salarius sp. nov.）。模式菌株为CL-Z59(T)（=KCCM 42320(T)=DSM 18239(T)）。
