Leary J F, Notter M F, Todd P
J Histochem Cytochem. 1976 Dec;24(12):1249-57. doi: 10.1177/24.12.187690.
Human cells in culture (HEp-2) were infected with herpes simplex virus type 2 (HSV-2) at multiplicities of infection varying from 0.2 to 10, and fixed 6, 12, 18 and 24 hr after infection. Infection-related antigens were detected by an indirect double antibody (peroxidase conjugated goat anti-rabbit to rabbit anti-herpes simplex virus type 2) immunoenzymatic staining reaction that rendered infection-related antigens visible by light microscopy. A corresponding series of laser flow cytophotometric experiments yielded reproducible large-angle (1-19 degrees) laser-light scattering distributions that depended upon multiplicities of infection and the location of the infection-related antigens in the infected cells.
将培养的人细胞（HEp-2）用2型单纯疱疹病毒（HSV-2）以感染复数从0.2至10进行感染，并在感染后6、12、18和24小时进行固定。通过间接双抗体（过氧化物酶偶联的山羊抗兔针对兔抗2型单纯疱疹病毒）免疫酶染色反应检测感染相关抗原，该反应通过光学显微镜使感染相关抗原可见。相应的一系列激光流式细胞光度实验产生了可重复的大角度（1-19度）激光散射分布，其取决于感染复数以及感染相关抗原在受感染细胞中的位置。
