Sabil D, Othman S K, Isahak I
Department of Microbiology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur.
Malays J Pathol. 1990 Jun;12(1):35-8.
150 specimens from suspected herpes simplex genital and skin lesions were received in virus transport medium. They were inoculated into Hep-2 cell monolayers, examined for the presence of virus by cytopathic effect (CPE), direct immunoperoxidase (DIP) and direct immunofluorescence (DIF). Of 39 (26.0%) virus-positive specimens by CPE, 37 (24.7%) were HSV-positive by DIP and 36 (24.0%) by DIF staining. DIP staining had a sensitivity of 100%, specificity of 99.1%, positive predictive value of 97.3% and negative predictive value of 100% in relation to DIF as a standard test. Of 39 specimens positive by CPE, only 25.6% were HSV-positive within 24 h post-inoculation compared to 94% HSV-positive by DIP and DIF staining at the same time.
150份来自疑似单纯疱疹生殖器和皮肤病变的标本被置于病毒运输培养基中。将它们接种到Hep-2单层细胞中，通过细胞病变效应（CPE）、直接免疫过氧化物酶（DIP）和直接免疫荧光（DIF）检测病毒的存在。在通过CPE检测为病毒阳性的39份（26.0%）标本中，通过DIP检测为HSV阳性的有37份（24.7%），通过DIF染色检测为阳性的有36份（24.0%）。以DIF作为标准检测，DIP染色的敏感性为100%，特异性为99.1%，阳性预测值为97.3%，阴性预测值为100%。在通过CPE检测为阳性的39份标本中，接种后24小时内只有25.6%为HSV阳性，而同时通过DIP和DIF染色检测为HSV阳性的比例为94%。
