Leary J F, Todd P
J Histochem Cytochem. 1977 Jul;25(7):908-12. doi: 10.1177/25.7.197163.
Human cells in culture (H.Ep. 2) were infected with herpes simplex virus type 2 at a multiplicity of infection (m.o.i.) of 3 for 3, 18 and 36 hr. Spatial redistribution of concanavalin (Con-A) cell surface receptor sites was detected by an indirect double-antibody (peroxidase-conjugated goat anti-rabbit to rabbit anti-Con-A) immunoenzymatic staining reaction which rendered the Con-A sites visible to light microscopy and to flow laser cytophotometry. Significant spatial redistribution of cell surface Con-A receptor sites occurred within the first 3 hr after infection with herpes simplex virus type 2. The infected cells must be in the presence of Con-A for the redistribution to occur, and significant redistribution occurs only at 37 degrees C. Fixation of infected cells prior to application of Con-A prevented this spatial redistribution of Con-A receptor sites.
将人培养细胞（H.Ep.2）以感染复数（m.o.i.）为3接种2型单纯疱疹病毒，分别培养3小时、18小时和36小时。通过间接双抗体（过氧化物酶标记的山羊抗兔-兔抗伴刀豆球蛋白A）免疫酶染色反应检测伴刀豆球蛋白A（Con-A）细胞表面受体位点的空间重新分布，该反应使Con-A位点在光学显微镜和流式激光细胞光度术中可见。在感染2型单纯疱疹病毒后的最初3小时内，细胞表面Con-A受体位点发生了显著的空间重新分布。感染细胞必须在有Con-A的情况下才会发生重新分布，且显著的重新分布仅在37℃时出现。在应用Con-A之前对感染细胞进行固定可防止Con-A受体位点的这种空间重新分布。
