淋巴细胞性脉络丛脑膜炎病毒（LCMV）：增殖、定量及保存
Lymphocytic choriomeningitis virus (LCMV): propagation, quantitation, and storage.
作者信息
Welsh Raymond M, Seedhom Mina O
机构信息
University of Massachusetts Medical School, Worcester, Massachusetts, USA.
出版信息
Curr Protoc Microbiol. 2008 Feb;Chapter 15:Unit 15A.1. doi: 10.1002/9780471729259.mc15a01s8.
Abstract
Lymphocytic choriomeningitis virus (LCMV) is an enveloped, ambisense RNA virus and the prototypic virus of the arenavirus group. It can cause viral meningitis and other ailments in humans, but its natural host is the mouse. The LCMV/mouse model has been useful for examining mechanisms of viral persistence and the basic concepts of virus-induced immunity and immunopathology. This unit discusses strain differences and biosafety containment issues for LCMV. Recommendations are made for techniques for propagating LCMV to high titers to quantify it by plaque assay and PCR techniques and to preserve its infectivity by appropriate storage.
摘要
淋巴细胞性脉络丛脑膜炎病毒（LCMV）是一种有包膜的双义RNA病毒，也是沙粒病毒属的原型病毒。它可导致人类患病毒性脑膜炎和其他疾病，但其天然宿主是小鼠。LCMV/小鼠模型对于研究病毒持续存在的机制以及病毒诱导的免疫和免疫病理学的基本概念很有用。本单元讨论了LCMV的毒株差异和生物安全控制问题。针对将LCMV增殖至高滴度以通过噬斑测定和PCR技术进行定量以及通过适当储存来保存其感染性的技术提出了建议。
相似文献
1
Lymphocytic choriomeningitis virus (LCMV): propagation, quantitation, and storage.淋巴细胞性脉络丛脑膜炎病毒（LCMV）：增殖、定量及保存
Curr Protoc Microbiol. 2008 Feb;Chapter 15:Unit 15A.1. doi: 10.1002/9780471729259.mc15a01s8.
2
Inhibition of multiplication of the prototypic arenavirus LCMV by valproic acid.丙戊酸抑制原型沙粒病毒 LCMV 的增殖。
Antiviral Res. 2013 Aug;99(2):172-9. doi: 10.1016/j.antiviral.2013.05.012. Epub 2013 Jun 2.
3
Analysis of the Function of the Lymphocytic Choriomeningitis Virus S Segment Untranslated Region on Growth Capacity In Vitro and on Virulence In Vivo.分析淋巴细胞性脉络丛脑膜炎病毒 S 片段非翻译区对体外生长能力和体内毒力的影响。
Viruses. 2020 Aug 16;12(8):896. doi: 10.3390/v12080896.
4
Residues K465 and G467 within the Cytoplasmic Domain of GP2 Play a Critical Role in the Persistence of Lymphocytic Choriomeningitis Virus in Mice.GP2胞质结构域内的K465和G467残基在淋巴细胞性脉络丛脑膜炎病毒在小鼠体内的持续存在中起关键作用。
J Virol. 2016 Oct 28;90(22):10102-10112. doi: 10.1128/JVI.01303-16. Print 2016 Nov 15.
5
Accelerated and improved quantification of lymphocytic choriomeningitis virus (LCMV) titers by flow cytometry.通过流式细胞术加速和改进淋巴细胞脉络丛脑膜炎病毒 (LCMV) 滴度的定量。
PLoS One. 2012;7(5):e37337. doi: 10.1371/journal.pone.0037337. Epub 2012 May 17.
6
Animal models using lymphocytic choriomeningitis virus.使用淋巴细胞性脉络丛脑膜炎病毒的动物模型。
Curr Protoc Immunol. 2001 May;Chapter 19:Unit 19.10. doi: 10.1002/0471142735.im1910s36.
7
Lymphocytic choriomeningitis arenavirus requires cellular COPI and AP-4 complexes for efficient virion production.淋巴细胞性脉络丛脑膜炎病毒需要细胞 COPI 和 AP-4 复合物来有效产生病毒粒子。
J Virol. 2024 Mar 19;98(3):e0200623. doi: 10.1128/jvi.02006-23. Epub 2024 Feb 9.
8
Inhibition of cellular entry of lymphocytic choriomeningitis virus by amphipathic DNA polymers.两亲性DNA聚合物对淋巴细胞性脉络丛脑膜炎病毒细胞进入的抑制作用
Virology. 2008 Mar 1;372(1):107-17. doi: 10.1016/j.virol.2007.10.016. Epub 2007 Nov 26.
9
Recombinant lymphocytic choriomeningitis virus expressing vesicular stomatitis virus glycoprotein.表达水疱性口炎病毒糖蛋白的重组淋巴细胞性脉络丛脑膜炎病毒
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7895-900. doi: 10.1073/pnas.1332709100. Epub 2003 Jun 13.
10
The small RING finger protein Z drives arenavirus budding: implications for antiviral strategies.小环状结构域蛋白Z驱动沙粒病毒出芽：对抗病毒策略的启示
Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12978-83. doi: 10.1073/pnas.2133782100. Epub 2003 Oct 16.
引用本文的文献
1
Early methionine availability attenuates T cell exhaustion.早期蛋氨酸的可利用性可减轻T细胞耗竭。
Nat Immunol. 2025 Jul 23. doi: 10.1038/s41590-025-02223-6.
2
Refined cell transfer model reveals roles for Ascl2 and Cxcr3 in splenic localization of mouse NK cells during virus infection.优化的细胞转移模型揭示了在病毒感染期间Ascl2和Cxcr3在小鼠自然杀伤细胞脾脏定位中的作用。
J Immunol. 2025 Jul 22. doi: 10.1093/jimmun/vkaf122.
3
Tim-3 Promotes Early Differentiation of Tbet Effector T Cells During Acute Viral Infection.Tim-3在急性病毒感染期间促进Tbet效应T细胞的早期分化。
bioRxiv. 2025 Jul 11:2025.07.08.663709. doi: 10.1101/2025.07.08.663709.
4
Polyclonal B cells acquire LCMV antigens in a GP1-dependent manner.多克隆B细胞以依赖GP1的方式获取淋巴细胞脉络丛脑膜炎病毒（LCMV）抗原。
PLoS Pathog. 2025 Jul 9;21(7):e1013345. doi: 10.1371/journal.ppat.1013345. eCollection 2025 Jul.
5
HDAC1 controls the generation and maintenance of effector-like CD8+ T cells during chronic viral infection.HDAC1在慢性病毒感染期间控制效应样CD8 + T细胞的生成和维持。
J Exp Med. 2025 Aug 4;222(8). doi: 10.1084/jem.20240829. Epub 2025 Jun 4.
6
Prothrombin prevents fatal T cell-dependent anemia during chronic virus infection of mice.凝血酶原可预防小鼠慢性病毒感染期间致命的T细胞依赖性贫血。
JCI Insight. 2025 Jan 16;10(4):e181063. doi: 10.1172/jci.insight.181063.
7
Type I interferon signaling in dendritic cells limits direct antigen presentation and CD8 T cell responses against an arthritogenic alphavirus.树突状细胞中的I型干扰素信号传导限制了直接抗原呈递以及针对致关节炎甲病毒的CD8 T细胞反应。
mBio. 2024 Dec 11;15(12):e0293024. doi: 10.1128/mbio.02930-24. Epub 2024 Nov 13.
8
The Multifaceted Roles of NK Cells in the Context of Murine Cytomegalovirus and Lymphocytic Choriomeningitis Virus Infections.自然杀伤细胞在小鼠巨细胞病毒和淋巴细胞性脉络丛脑膜炎病毒感染背景下的多方面作用
Immune Netw. 2024 Jun 27;24(4):e29. doi: 10.4110/in.2024.24.e29. eCollection 2024 Aug.
9
Detection of Lymphocytic Choriomeningitis Virus-Specific Memory B Cells Using Antigen Tetramers.使用抗原四聚体检测淋巴细胞性脉络丛脑膜炎病毒特异性记忆 B 细胞。
Methods Mol Biol. 2024;2826:117-129. doi: 10.1007/978-1-0716-3950-4_9.
10
Type I interferon induced during chronic viral infection favors B-cell development in the thymus.慢性病毒感染期间诱导的 I 型干扰素有利于胸腺中的 B 细胞发育。
Immunol Cell Biol. 2024 Oct;102(9):801-816. doi: 10.1111/imcb.12808. Epub 2024 Jul 15.
本文引用的文献
1
Signaling through Itk promotes T helper 2 differentiation via negative regulation of T-bet.通过Itk发出的信号通过对T-bet的负调控促进辅助性T细胞2分化。
Immunity. 2004 Jul;21(1):67-80. doi: 10.1016/j.immuni.2004.06.009.
2
CD1d1-dependent control of the magnitude of an acute antiviral immune response.CD1d1依赖性对急性抗病毒免疫反应强度的控制。
J Immunol. 2004 Mar 15;172(6):3454-61. doi: 10.4049/jimmunol.172.6.3454.
3
Reverse genetics of arenaviruses.沙粒病毒的反向遗传学
Curr Top Microbiol Immunol. 2002;262:175-93. doi: 10.1007/978-3-642-56029-3_8.
4
Viral persistence in vivo through selection of neutralizing antibody-escape variants.通过选择中和抗体逃逸变体在体内实现病毒持续存在。
Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2749-54. doi: 10.1073/pnas.040558797.
5
Viral immune evasion due to persistence of activated T cells without effector function.由于无效应功能的活化T细胞持续存在导致的病毒免疫逃逸。
J Exp Med. 1998 Dec 21;188(12):2205-13. doi: 10.1084/jem.188.12.2205.
6
Identification of alpha-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus.鉴定α- dystroglycan作为淋巴细胞性脉络丛脑膜炎病毒和拉沙热病毒的受体。
Science. 1998 Dec 11;282(5396):2079-81. doi: 10.1126/science.282.5396.2079.
7
Virus persistence in acutely infected immunocompetent mice by exhaustion of antiviral cytotoxic effector T cells.通过耗尽抗病毒细胞毒性效应T细胞，病毒在急性感染的免疫健全小鼠中持续存在。
Nature. 1993 Apr 22;362(6422):758-61. doi: 10.1038/362758a0.
8
Virus-induced immunosuppression: kinetic analysis of the selection of a mutation associated with viral persistence.病毒诱导的免疫抑制：与病毒持续存在相关的突变选择的动力学分析
J Virol. 1994 Nov;68(11):7367-73. doi: 10.1128/JVI.68.11.7367-7373.1994.
9
CTL escape viral variants. I. Generation and molecular characterization.细胞毒性T淋巴细胞逃逸病毒变体。I. 产生及分子特征
Virology. 1995 Jun 20;210(1):29-40. doi: 10.1006/viro.1995.1314.
10
Inhibition of immunologic injury of cultured cells infected with lymphocytic choriomeningitis virus: role of defective interfering virus in regulating viral antigenic expression.抑制淋巴细胞性脉络丛脑膜炎病毒感染的培养细胞的免疫损伤：缺陷干扰病毒在调节病毒抗原表达中的作用。
J Exp Med. 1977 Jun 1;145(6):1449-68. doi: 10.1084/jem.145.6.1449.
Zotero 插件