Welsh Raymond M, Seedhom Mina O
University of Massachusetts Medical School, Worcester, Massachusetts, USA.
Curr Protoc Microbiol. 2008 Feb;Chapter 15:Unit 15A.1. doi: 10.1002/9780471729259.mc15a01s8.
Lymphocytic choriomeningitis virus (LCMV) is an enveloped, ambisense RNA virus and the prototypic virus of the arenavirus group. It can cause viral meningitis and other ailments in humans, but its natural host is the mouse. The LCMV/mouse model has been useful for examining mechanisms of viral persistence and the basic concepts of virus-induced immunity and immunopathology. This unit discusses strain differences and biosafety containment issues for LCMV. Recommendations are made for techniques for propagating LCMV to high titers to quantify it by plaque assay and PCR techniques and to preserve its infectivity by appropriate storage.
淋巴细胞性脉络丛脑膜炎病毒（LCMV）是一种有包膜的双义RNA病毒，也是沙粒病毒属的原型病毒。它可导致人类患病毒性脑膜炎和其他疾病，但其天然宿主是小鼠。LCMV/小鼠模型对于研究病毒持续存在的机制以及病毒诱导的免疫和免疫病理学的基本概念很有用。本单元讨论了LCMV的毒株差异和生物安全控制问题。针对将LCMV增殖至高滴度以通过噬斑测定和PCR技术进行定量以及通过适当储存来保存其感染性的技术提出了建议。
