Varga Tamás, Palkovits Miklós, Usdin Ted Björn, Dobolyi Arpád
Neuromorphological and Neuroendocrine Research Laboratory, Department of Anatomy, Histology and Embryology, Semmelweis University and the Hungarian Academy of Sciences, Budapest, Hungary.
J Comp Neurol. 2008 Nov 10;511(2):221-37. doi: 10.1002/cne.21829.
Previously, we described a cell group expressing tuberoinfundibular peptide of 39 residues (TIP39) in the lateral pontomesencephalic tegmentum, and referred to it as the medial paralemniscal nucleus (MPL). To identify this nucleus further in rat, we have now characterized the MPL cytoarchitectonically on coronal, sagittal, and horizontal serial sections. Neurons in the MPL have a columnar arrangement distinct from adjacent areas. The MPL is bordered by the intermediate nucleus of the lateral lemniscus nucleus laterally, the oral pontine reticular formation medially, and the rubrospinal tract ventrally, whereas the A7 noradrenergic cell group is located immediately mediocaudal to the MPL. TIP39-immunoreactive neurons are distributed throughout the cytoarchitectonically defined MPL and constitute 75% of its neurons as assessed by double labeling of TIP39 with a fluorescent Nissl dye or NeuN. Furthermore, we investigated the neuronal inputs to the MPL by using the retrograde tracer cholera toxin B subunit. The MPL has afferent neuronal connections distinct from adjacent brain regions including major inputs from the auditory cortex, medial part of the medial geniculate body, superior colliculus, external and dorsal cortices of the inferior colliculus, periolivary area, lateral preoptic area, hypothalamic ventromedial nucleus, lateral and dorsal hypothalamic areas, subparafascicular and posterior intralaminar thalamic nuclei, periaqueductal gray, and cuneiform nucleus. In addition, injection of the anterograde tracer biotinylated dextran amine into the auditory cortex and the hypothalamic ventromedial nucleus confirmed projections from these areas to the distinct MPL. The afferent neuronal connections of the MPL suggest its involvement in auditory and reproductive functions.
先前，我们描述了在脑桥中脑外侧被盖区表达39个氨基酸的结节漏斗肽（TIP39）的细胞群，并将其称为内侧旁臂核（MPL）。为了在大鼠中进一步鉴定该核团，我们现在在冠状、矢状和水平连续切片上对MPL进行了细胞构筑学特征分析。MPL中的神经元呈柱状排列，与相邻区域不同。MPL的外侧与外侧丘系中间核相邻，内侧与脑桥嘴侧网状结构相邻，腹侧与红核脊髓束相邻，而A7去甲肾上腺素能细胞群位于MPL的紧邻中间尾侧。通过用荧光尼氏染料或NeuN对TIP39进行双重标记评估，TIP39免疫反应性神经元分布在细胞构筑学定义的整个MPL中，占其神经元的75%。此外，我们使用逆行示踪剂霍乱毒素B亚单位研究了MPL的神经元输入。MPL具有与相邻脑区不同的传入神经元连接，包括来自听觉皮层、内侧膝状体内侧部分、上丘、下丘的外侧和背侧皮层、橄榄周区域、外侧视前区、下丘脑腹内侧核、下丘脑外侧和背侧区域、束旁下核和丘脑板内核、导水管周围灰质和楔形核的主要输入。此外，将顺行示踪剂生物素化葡聚糖胺注入听觉皮层和下丘脑腹内侧核证实了从这些区域到不同MPL的投射。MPL的传入神经元连接表明其参与听觉和生殖功能。
