1型单纯疱疹病毒对细胞DNA依赖性RNA聚合酶活性的影响。

The effects of herpes simplex virus type 1 on cellular DNA-dependent RNA polymerase activities.

作者信息

Preston C M, Newton A A

出版信息

J Gen Virol. 1976 Dec;33(3):471-82. doi: 10.1099/0022-1317-33-3-471.

Abstract

An investigation of the activity of nuclear RNA polymerase following infection of LS cells with HSV-1 shows a decline in both major activities. This effect is not entirely due to inhibition of cellular protein synthesis, and the effect of alpha-amanitin-sensitive RNA polymerase is mediated by a protein(s) synthesized in the infected cell. Changes in the properties of this RNA polymerase activity include a reduction in the relative UTP/GTP incorporation ratio and an increased sensitivity to inhibition by actinomycin D, indicating that RNA polymerase II is involved in virus transcription.

摘要

对单纯疱疹病毒1型（HSV - 1）感染LS细胞后核RNA聚合酶活性的研究表明，两种主要活性均有所下降。这种效应并非完全归因于细胞蛋白质合成的抑制，且α - 鹅膏蕈碱敏感的RNA聚合酶的效应是由感染细胞中合成的一种或多种蛋白质介导的。这种RNA聚合酶活性特性的变化包括相对UTP / GTP掺入率降低以及对放线菌素D抑制的敏感性增加，这表明RNA聚合酶II参与病毒转录。