Costanzo F, Campadelli-Fiume G, Foa-Tomasi L, Cassai E
J Virol. 1977 Mar;21(3):996-1001. doi: 10.1128/JVI.21.3.996-1001.1977.
In herpes simplex virus type 1 (HSV-1)-infected HEp-2 cells, amanitin added before or at various times after infection always reduced viral multiplication. Also, the three waves of transcription of HSV-1 DNA, which led to the synthesis of alpha, beta-, and gamma-polypeptides, were all sensitive to amanitin in HEp-2 cells, and the amanitin-sensitive RNA polymerase activities of isolated nuclei were equally sensitive to the inhibitor before and during the infection. On the contrary, HSV-1 DNA transcription was totally unaffected by amanitin in AR1/9-5B cells, a mutant subline of CHO cells that possesses an amanitin-resistant RNA polymerase B. Together, these results strongly suggest that HSV-1 DNA utilizes for its transcription a polymerase undistinguishable from host cell RNA polymerase B with respect to its sensitivity to amanitin.
在单纯疱疹病毒1型（HSV-1）感染的人喉表皮癌细胞（HEp-2细胞）中，在感染前或感染后不同时间添加鹅膏蕈碱总是会减少病毒增殖。此外，HSV-1 DNA的三波转录导致α、β和γ多肽的合成，在HEp-2细胞中这三波转录均对鹅膏蕈碱敏感，并且在感染前和感染期间，分离细胞核中对鹅膏蕈碱敏感的RNA聚合酶活性对该抑制剂同样敏感。相反，在AR1/9-5B细胞（中国仓鼠卵巢细胞的一个突变亚系，具有对鹅膏蕈碱耐药的RNA聚合酶B）中，HSV-1 DNA转录完全不受鹅膏蕈碱影响。这些结果共同强烈表明，HSV-1 DNA转录所利用的聚合酶在对鹅膏蕈碱的敏感性方面与宿主细胞RNA聚合酶B无法区分。
