单纯疱疹病毒特异性α蛋白与宿主细胞RNA聚合酶II在病毒脱氧嘧啶激酶转录过程中的合作。

Cooperation between herpes simplex virus specific alpha protein and host cell RNA polymerase II in the transcription of viral deoxypyrimidine kinase.

作者信息

Leung W C

出版信息

Can J Microbiol. 1980 Mar;26(3):401-4. doi: 10.1139/m80-066.

Abstract

A cistron specific enzyme-forming capacity method was used to study the control of herpes simplex virus (HSV) specific deoxypyrimidine kinase (dPyK) mRNA synthesis. In this assay, the alpha (or immediately early) protein was required to effect the transcription of dPyK mRNA. However, the dPyK mRNA synthesis was sensitive to alpha-amanitin in alpha-amanitin sensitive cells and resistant to alpha-amanitin in alpha-amanitin resistant cells. The effective dose range of alpha-amanitin used and the genetic lesion in alpha-amanitin resistant cells suggested that cellular DNA-dependent RNA polymerase II was also involved in the transcription of dPyK. This study suggests that two components, the HSV alpha protein and the cellular RNA polymerase, II, were required for dPyK mRNA synthesis.

摘要

采用顺反子特异性酶形成能力方法研究单纯疱疹病毒（HSV）特异性脱氧嘧啶激酶（dPyK）mRNA合成的调控。在该测定中，α（或即刻早期）蛋白是影响dPyK mRNA转录所必需的。然而，dPyK mRNA合成在α-鹅膏蕈碱敏感细胞中对α-鹅膏蕈碱敏感，而在α-鹅膏蕈碱抗性细胞中对α-鹅膏蕈碱具有抗性。所使用的α-鹅膏蕈碱的有效剂量范围以及α-鹅膏蕈碱抗性细胞中的遗传损伤表明，细胞DNA依赖性RNA聚合酶II也参与了dPyK的转录。这项研究表明，dPyK mRNA合成需要两种成分，即HSVα蛋白和细胞RNA聚合酶II。