Stenberg R M, Pizer L I
J Virol. 1982 May;42(2):474-87. doi: 10.1128/JVI.42.2.474-487.1982.
We used the viral transcripts (designated Ad-RNA) that accumulated in the cytoplasm of adenovirus type 5-transformed human embryonic kidney cells (cell line 291-31) as models for cellular RNAs to examine how herpes simplex virus modifies cellular RNA metabolism. Infection of 293-31 cells with herpes simplex virus type 1 strain 17 lead to extensive inhibition of Ad-RNA accumulation by 4 h postinfection. The major part of this inhibition was due to an immediate early or alpha gene function, which reduced the rate of transcription of Ad-RNA within the nuclei of the infected cells. In addition, host polyadenylic acid-containing RNA accumulation and rRNA accumulation were affected, but to a lesser extent and at lower rate than Ad-RNA accumulation. In conjunction with previous data, our experimental data allowed us to propose a general scheme for how herpes simplex virus type 1 alters the metabolism of cellular RNA, the possible mechanisms for these changes, and how they correlate with the regulation of herpes simplex virus gene expression.
我们使用在5型腺病毒转化的人胚肾细胞（291 - 31细胞系）细胞质中积累的病毒转录本（称为腺病毒RNA，Ad - RNA）作为细胞RNA的模型，来研究单纯疱疹病毒如何改变细胞RNA代谢。用1型单纯疱疹病毒17株感染293 - 31细胞，在感染后4小时导致Ad - RNA积累受到广泛抑制。这种抑制的主要部分归因于即刻早期或α基因功能，它降低了受感染细胞核内Ad - RNA的转录速率。此外，宿主含聚腺苷酸的RNA积累和rRNA积累也受到影响，但程度较小且速率低于Ad - RNA积累。结合先前的数据，我们的实验数据使我们能够提出一个关于1型单纯疱疹病毒如何改变细胞RNA代谢、这些变化的可能机制以及它们如何与单纯疱疹病毒基因表达调控相关的总体方案。
