翻译绕过而不通过肽基 - tRNA对编码间隙mRNA进行反密码子扫描。
Translational bypassing without peptidyl-tRNA anticodon scanning of coding gap mRNA.
作者信息
Wills Norma M, O'Connor Michelle, Nelson Chad C, Rettberg Charles C, Huang Wai Mun, Gesteland Raymond F, Atkins John F
机构信息
Department of Human Genetics, University of Utah, Salt Lake City, UT, USA.
出版信息
EMBO J. 2008 Oct 8;27(19):2533-44. doi: 10.1038/emboj.2008.170. Epub 2008 Sep 4.
Abstract
Half the ribosomes translating the mRNA for phage T4 gene 60 topoisomerase subunit bypass a 50 nucleotide coding gap between codons 46 and 47. The pairing of codon 46 with its cognate peptidyl-tRNA anticodon dissociates, and following mRNA slippage, peptidyl-tRNA re-pairs to mRNA at a matched triplet 5' adjacent to codon 47, where translation resumes. Here, in studies with gene 60 cassettes, it is shown that the peptidyl-tRNA anticodon does not scan the intervening sequence for potential complementarity. However, certain coding gap mutants allow peptidyl-tRNA to scan sequences in the bypassed segment. A model is proposed in which the coding gap mRNA enters the ribosomal A-site and forms a structure that precludes peptidyl-tRNA scanning of its sequence. Dissipation of this RNA structure, together with the contribution of 16S rRNA anti-Shine-Dalgarno sequence pairing with GAG, facilitates peptidyl-tRNA re-pairing to mRNA.
摘要
翻译噬菌体T4基因60拓扑异构酶亚基mRNA的核糖体中有一半会绕过密码子46和47之间50个核苷酸的编码间隙。密码子46与其同源肽基-tRNA反密码子的配对解离，在mRNA滑动后，肽基-tRNA在与密码子47相邻的5'端匹配三联体处重新与mRNA配对，翻译在此处恢复。在此，在对基因60盒式结构的研究中表明，肽基-tRNA反密码子不会扫描间隔序列以寻找潜在的互补性。然而，某些编码间隙突变体允许肽基-tRNA扫描被绕过片段中的序列。提出了一个模型，其中编码间隙mRNA进入核糖体A位点并形成一种结构，该结构阻止肽基-tRNA对其序列进行扫描。这种RNA结构的消散，以及16S rRNA反-Shine-Dalgarno序列与GAG配对的作用，促进了肽基-tRNA与mRNA的重新配对。
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