Sladek Zbysek, Rysanek Dusan
Department of Morphology, Mendel University of Agriculture and Forestry, Zemedelská 1, Brno 613 00, Czech Republic.
Res Vet Sci. 2009 Apr;86(2):235-40. doi: 10.1016/j.rvsc.2008.07.016. Epub 2008 Sep 6.
The aim of this study was to investigate development over time of the surface expression of CD44 on macrophages during an inflammatory response of bovine mammary gland. Intramammary instillation of muramyl dipeptide (MDP) and lipopolysaccharide (LPS) resulted in a significant increase in the total count of CD44+ non-vacuolised macrophages ((N)MAC) after 24h. During resolution of the inflammatory response, there was observed a gradual decrease in the total count CD44+ (N)MAC. The lower total count and proportion of CD44+vacuolised macrophages ((V)MAC) was observed as the effect of MDP and LPS at 24h after induction (P<0.01). During resolution, the total count and proportion of CD44+(V)MAC increased. We have demonstrated CD44 receptor is expressed during the inflammatory response caused by LPS and MDP and the effect of these components on CD44 expression was particularly evident during initiation of the inflammatory response. High expression of CD44 in resolution of inflammatory response may relate to macrophages involvement in the processes leading to restitution of injured tissues.
本研究的目的是调查在牛乳腺炎症反应期间巨噬细胞上CD44表面表达随时间的变化情况。乳房内注射胞壁酰二肽（MDP）和脂多糖（LPS）导致24小时后CD44+非空泡化巨噬细胞（(N)MAC）总数显著增加。在炎症反应消退过程中，观察到CD44+(N)MAC总数逐渐减少。诱导后24小时，观察到MDP和LPS作用下CD44+空泡化巨噬细胞（(V)MAC）总数和比例较低（P<0.01）。在消退过程中，CD44+(V)MAC总数和比例增加。我们已经证明，在由LPS和MDP引起的炎症反应期间，CD44受体表达，并且这些成分对CD44表达的影响在炎症反应开始时尤为明显。炎症反应消退过程中CD44的高表达可能与巨噬细胞参与导致受损组织恢复的过程有关。
