Slama Petr, Kabourkova Eliska, Sladek Zbysek, Zavadilova Terezie, Kratochvilova Lucie, Kharkevich Kristina, Roychoudhury Shubhadeep, Pavlik Ales, Roztocilova Andrea, Uhrincat Michal, Tancin Vladimir, Kimura Kazuhiro, Konecny Roman, Kiku Yoshio, Watanabe Atsushi, Kwak Jong-Young, Zouharova Monika
Department of Animal Morphology, Physiology and Genetics, Faculty of AgriSciences, Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech Republic.
Department of Animal Origin Food and Gastronomic Sciences, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1, 612 42 Brno, Czech Republic.
Animals (Basel). 2020 Jun 5;10(6):990. doi: 10.3390/ani10060990.
The aim of this study was to evaluate whether apoptosis of lymphocytes is modulated by stimulation by lipopolysaccharide (LPS) of or muramyl dipeptide (MDP). Cell populations were obtained by lavaging of the mammary glands 24, 48, 72, and 168 hours following intramammary induced inflammation. The portion of apoptotic lymphocytes peaked at 48 hours after treatment with LPS or MDP. The analysis of CD44 expression of the same cell populations showed a higher percentage of CD44-positive lymphocytes 24- and 48-hours following induction of inflammation by LPS or MDP. The results demonstrate that during both experimental infection of bovine mammary glands with LPS or MDP, apoptosis of lymphocytes was induced in the initial phase of the inflammatory response and CD44 was also overexpressed at the beginning of inflammation. These data suggest a connection of lymphocyte apoptosis with the expression of CD44 receptors.
本研究的目的是评估淋巴细胞凋亡是否受脂多糖（LPS）或胞壁酰二肽（MDP）刺激的调节。在乳腺内诱导炎症后24、48、72和168小时，通过灌洗乳腺获得细胞群体。在用LPS或MDP处理后，凋亡淋巴细胞的比例在48小时达到峰值。对相同细胞群体的CD44表达分析显示，在LPS或MDP诱导炎症后24小时和48小时，CD44阳性淋巴细胞的百分比更高。结果表明，在用LPS或MDP对牛乳腺进行实验性感染期间，炎症反应的初始阶段诱导了淋巴细胞凋亡，并且在炎症开始时CD44也过度表达。这些数据表明淋巴细胞凋亡与CD44受体的表达之间存在联系。
