Mochizuki Kazufumi
IMBA (Institute of Molecular Biotechnology of the Austrian Academy of Sciences), Dr. Bohr-Gasse 3, A-1030 Vienna, Austria.
Gene. 2008 Dec 1;425(1-2):79-83. doi: 10.1016/j.gene.2008.08.007. Epub 2008 Aug 15.
Tetrahymena thermophila is a useful model for the study of eukaryotic biology. A neomycin resistance gene (neo) has been developed that was optimized for the codon usage of T. thermophila. Using this codon-optimized neo gene (neoTet), a new drug resistance marker cassette, neo4, has been constructed. The neo4 cassette resulted in about ten times more drug resistant transformants than a cassette containing the non-codon-optimized original neo gene. The new cassette enables transgenic Tetrahymena strains to be created with high efficiency. This study also emphasizes the importance of codon optimization in transgene expression in Tetrahymena.
嗜热四膜虫是研究真核生物生物学的有用模型。已开发出一种针对嗜热四膜虫密码子使用情况进行优化的新霉素抗性基因（neo）。利用这个密码子优化的neo基因（neoTet），构建了一个新的耐药性标记盒neo4。与包含未进行密码子优化的原始neo基因的盒相比，neo4盒产生的耐药转化体数量多出约十倍。这个新的盒能够高效创建转基因嗜热四膜虫菌株。这项研究还强调了密码子优化在嗜热四膜虫转基因表达中的重要性。
