Santos Adriana O, Ueda-Nakamura Tânia, Dias Filho Benedito P, Veiga Junior Valdir F, Pinto Angelo C, Nakamura Celso Vataru
Programa de Pós-Graduação em Microbiologia, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid s/n, 86051-990 Londrina-PR, Brazil.
J Ethnopharmacol. 2008 Nov 20;120(2):204-8. doi: 10.1016/j.jep.2008.08.007. Epub 2008 Aug 15.
Copaiba oil has been used in folk medicine since the 19th century. The use of copaiba oils to treat leishmaniasis is cited in several ethnopharmacological studies. Nevertheless, the potential antileishmania of copaiba oils had not been studied.
Eight different kinds of Brazilian copaiba oils were screened for antileishmanial activity.
The antiproliferative effect of copaiba oil on promastigote and amastigote axenic were determined. To determine the survival index peritoneal macrophage were infected with promastigotes of Leishmania amazonensis and treated with copaiba oil. The cytotoxic effect of copaiba oil was assessed on macrophage strain J774G8 by assay of sulforhodamine B.
Copaiba oils showed variable levels of activity against promastigote forms with IC(50) values in the range between 5 and 22microg/mL. The most active oil was that from Copaifera reticulata (collected in Pará State, Brazil) with IC(50) values of 5, 15, and 20microg/mL for promastigote, axenic amastigote and intracellular amastigote forms, respectively. Amphotericin B showed IC(50) of 0.058 and 0.231microg/mL against promastigote and amastigote forms, respectively. Cytotoxicity assay showed that this copaiba oil obtained from Copaifera reticulata showed low cytotoxicity against J774G8 macrophages.
Copaiba oils showed significant activity against the parasite Leishmania amazonensis.
自19世纪以来，古巴香脂油就已用于民间医学。多项民族药理学研究提到了使用古巴香脂油治疗利什曼病。然而，古巴香脂油潜在的抗利什曼原虫作用尚未得到研究。
对八种不同的巴西古巴香脂油进行抗利什曼原虫活性筛选。
测定古巴香脂油对前鞭毛体和无鞭毛体体外培养的抗增殖作用。为了确定存活指数，将亚马逊利什曼原虫的前鞭毛体感染腹腔巨噬细胞，并用古巴香脂油进行处理。通过磺酰罗丹明B测定法评估古巴香脂油对巨噬细胞系J774G8的细胞毒性作用。
古巴香脂油对前鞭毛体形式显示出不同程度的活性，IC50值在5至22微克/毫升之间。活性最强的油来自网脉古柯（采自巴西帕拉州），对前鞭毛体、无鞭毛体体外培养和细胞内无鞭毛体形式的IC50值分别为5、15和20微克/毫升。两性霉素B对前鞭毛体和无鞭毛体形式的IC50分别为0.058和0.231微克/毫升。细胞毒性测定表明，从网脉古柯获得的这种古巴香脂油对J774G8巨噬细胞的细胞毒性较低。
古巴香脂油对亚马逊利什曼原虫显示出显著活性。
