细菌核糖核酸酶P识别底物的亚位点。

Subsites for substrate recognition by bacterial ribonuclease P.

作者信息

Fujimoto Akihiro, Suwa Satoshi, Nagai Yasuhiro, Kikuchi Yo, Tanaka Terumichi

机构信息

Tempakucho, Toyohashi, Aichi 441-8580, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):207-8. doi: 10.1093/nass/nrn105.

DOI:10.1093/nass/nrn105

PMID:18776326

Abstract

We have prepared series of shape variant RNAs of a tRNA precursor and analyzed the substrate shape recognition by bacterial ribonuclease P ribozyme and holoenzyme. The results showed the evidence for the presence of subsites for the recognition of the trna shape. We will discuss a new model for substrate recognition and the role of the protein component.

摘要

我们制备了一系列tRNA前体的形状变体RNA，并分析了细菌核糖核酸酶P核酶和全酶对底物形状的识别。结果显示了存在用于识别tRNA形状的亚位点的证据。我们将讨论一种新的底物识别模型以及蛋白质组分的作用。