用于单核苷酸多态性检测的新型基于准分子的串联系统。

New eximer-based tandem systems for SNP detection.

作者信息

Novopashina Daria S, Meschaninova Maria I, Kholodar Svetlana A, Lomzov Alexander A, Venyaminova Alya G

机构信息

Institute of Chemical Biology and Fundamental Medicine SB RAS, 8 Lavrentiev ave., Novosibirsk 630090, Russia.

出版信息

Nucleic Acids Symp Ser (Oxf). 2008(52):229-30. doi: 10.1093/nass/nrn116.

DOI:10.1093/nass/nrn116

PMID:18776337

Abstract

We report here the design and synthesis of new mono- and bis-pyrene-labeled oligo(2'-O-methylribonucleotide) tandems as perspective probes for SNP detection. The detection strategy is based on the eximer formation when two or more pyrene groups are brought into close proximity upon hybridization of the tandem components with DNA. The potential of SNP detection with tandems of pyrene-labeled oligo(2'-O-methylribonucleotides) by duplex melting curve analysis based on excimer fluorescence registration was demonstrated.

摘要

我们在此报告新型单芘和双芘标记的寡聚（2'-O-甲基核糖核苷酸）串联体的设计与合成，作为用于单核苷酸多态性（SNP）检测的前景探针。检测策略基于当串联体组分与DNA杂交时两个或更多芘基团紧密靠近形成准分子。基于准分子荧光记录，通过双链体解链曲线分析证明了芘标记的寡聚（2'-O-甲基核糖核苷酸）串联体用于SNP检测的潜力。