Terato Hiroaki, Watari Hiromi, Shimazaki Yuka, Hirayama Ryoichi, Furusawa Yoshiya, Ide Hiroshi
Analytical Research Center for Experimental Sciences, Saga University, 5-1-1 Nabeshima, Saga 849- 8501, Japan.
Nucleic Acids Symp Ser (Oxf). 2008(52):443-4. doi: 10.1093/nass/nrn225.
Among numerous DNA damaging factors, ionizing radiation produces the damage showing very unique structure. Since ionizing radiation passes through a target DNA as a beam, the respective induced lesions locate close together around the track. Such damage aggregation on target DNA called "clustered DNA damage" is thought to be a major cause of the specific and serious effect of ionizing radiation. However, we have less knowledge about the structure of clustered DNA damage, which seems very important in its biological impact. Therefore, we evaluated procedure to analyze the structure of clustered DNA damage induced by ionizing radiation. In the present study, we used polyacrylamide gel electrophoresis to separate oligodeoxyribonucleotide (ODN) substrates containing clustered DNA damage with post-modification with aldehyde reactive probe. The designed procedure well counted the number of abasic site in the model substrate of clustered DNA damage.
在众多DNA损伤因素中，电离辐射产生的损伤呈现出非常独特的结构。由于电离辐射以束状穿过目标DNA，各个诱导损伤在轨迹周围紧密相邻。这种在目标DNA上的损伤聚集被称为“簇状DNA损伤”，被认为是电离辐射产生特定严重效应的主要原因。然而，我们对簇状DNA损伤的结构了解较少，而其结构在生物学影响方面似乎非常重要。因此，我们评估了分析电离辐射诱导产生的簇状DNA损伤结构的方法。在本研究中，我们使用聚丙烯酰胺凝胶电泳来分离含有经醛反应性探针后修饰的簇状DNA损伤的寡脱氧核糖核苷酸（ODN）底物。所设计的方法能很好地计算簇状DNA损伤模型底物中无碱基位点的数量。
