Bulow V V, Biggs P M
Houghton Poultry Research Station, Houghton, Huntingdon, Cambs., U.K.
Avian Pathol. 1975;4(2):133-46. doi: 10.1080/03079457509353859.
Two serological types of Marek's disease virus and a herpesvirus of turkeys have been differentiated by indirect immunofluorescence tests as (1) pathogenic strains of Marek's disease virus (MDV) and their attenuated variants: HPRS-16, HPRS-16/att, HPRS-B14, JM, JM/att, GA, VC and 'Oldenburg', a recent field isolate; (2) apathogenic strains HPRS-24 and HPRS-27 of MDV; (3) herpesvirus of turkeys strain FC126 and its HVT(A-) variant. Virus strains could not be distinguished on the basis of qualitative differences in immunofluorescent staining of intracellular virus-induced antigens. Results were similar whether chicken kidney, chicken embryo fibroblast or duck embryo fibroblast cell cultures were used. Fluorescence of virus-induced antigens was stronger with homologous than with heterologous antisera. Using the direct immunofluorescence technique Marek's disease virus and turkey herpesvirus infections could be distinguished. There were never any significant differences in the appearance and distribution of antigen in infected cells treated with homologous or heterologous antisera at dilutions of comparable activity using the indirect immunofluorescence technique. Antibody titres of antisera were 4 to 8-fold higher in the indirect immunofluorescence test against the homologous virus-induced antigens than against heterologous antigens. Cross-reactions between the 3 serological types could be prevented by absorption of antisera with the appropriate antigens. Cross-reactions could also be prevented by the appropriate dilution of antisera before use in the indirect immunofluorescence test.
通过间接免疫荧光试验已区分出两种血清型的马立克氏病病毒和一种火鸡疱疹病毒，分别为：(1) 马立克氏病病毒（MDV）的致病株及其减毒变种：HPRS - 16、HPRS - 16/att、HPRS - B14、JM、JM/att、GA、VC 以及“奥尔登堡”（一种近期的野外分离株）；(2) MDV的无致病力株HPRS - 24和HPRS - 27；(3) 火鸡疱疹病毒株FC126及其HVT(A -)变种。基于细胞内病毒诱导抗原免疫荧光染色的定性差异无法区分病毒株。无论使用鸡肾、鸡胚成纤维细胞还是鸭胚成纤维细胞培养物，结果均相似。病毒诱导抗原与同源抗血清的荧光反应强于与异源抗血清的反应。使用直接免疫荧光技术可区分马立克氏病病毒感染和火鸡疱疹病毒感染。在使用间接免疫荧光技术且以具有可比活性的稀释度用同源或异源抗血清处理的感染细胞中，抗原的外观和分布从未有任何显著差异。在间接免疫荧光试验中，抗血清针对同源病毒诱导抗原的抗体效价比针对异源抗原的高4至8倍。通过用适当抗原吸收抗血清，可防止3种血清型之间的交叉反应。在间接免疫荧光试验中使用前通过适当稀释抗血清也可防止交叉反应。
