Bulow V V, Biggs P M
Houghton Poultry Research Station, Houghton, Huntingdon, Cambs., U.K.
Avian Pathol. 1975;4(2):147-62. doi: 10.1080/03079457509353860.
Precipitating antigens associated with a number of Marek's disease virus strains and with a turkey herpesvirus have been analyzed. The 'A' antigen has been defined as the major soluble antigen in feather follicles of infected chickens, which is identical with the major antigen usually present in supernatants of chicken kidney cell cultures infected with strains of Marek's disease virus. 'BC' antigens are 2 or more antigens which are usually not noted in skin extracts but present in cultured cells infected with Marek's disease virus or turkey herpesvirus, in addition to the 'A' antigen. Some of the virus strains examined were positive and others negative for 'A' antigen, but all contained the 'BC' antigens. Results of agar-gel precipitin tests suggested a serological classification of the group of avian herpesviruses formed by Marek's disease viruses and turkey herpesvirus into 3 types. Pathogenic strains of Marek's disease virus and their attenuated A- variants, represented by the HPRS-16 strain (HPRS-16, JM, GA, VC, Oldenburg). Apathogenic Marek's disease virus, represented by the HPRS-24 strain. Turkey herpesvirus and its A- variants, represented by the FC126 strain. A serological subdivision corresponding to the different grades of pathogenicity of virus strains of the first type was not possible. Differences between antigens associated with the 3 types of virus were apparent from the antigen and antibody titres against homologous and heterologous reagents. Precipitin bands produced by homologous antigen and antibody were stronger than those produced by heterologous reagents. Differences between 'A' antigens of the 3 virus types were characterized by spur patterns of precipitin bands indicating a partial identity. At least 3 'BC' precipitin bands were noted; at least one was group-specific and one appeared to be type-specific.
已对与多种马立克氏病病毒株及火鸡疱疹病毒相关的沉淀抗原进行了分析。“A”抗原被定义为感染鸡羽毛滤泡中的主要可溶性抗原，它与通常存在于感染马立克氏病病毒株的鸡肾细胞培养上清液中的主要抗原相同。“BC”抗原是2种或更多种抗原，通常在皮肤提取物中未被发现，但除“A”抗原外，存在于感染马立克氏病病毒或火鸡疱疹病毒的培养细胞中。所检测的一些病毒株“A”抗原呈阳性，另一些呈阴性，但所有病毒株都含有“BC”抗原。琼脂凝胶沉淀试验结果表明，由马立克氏病病毒和火鸡疱疹病毒组成的禽疱疹病毒组在血清学上可分为3型。马立克氏病病毒的致病株及其减毒A变体，以HPRS - 16株（HPRS - 16、JM、GA、VC、奥尔登堡）为代表。以HPRS - 24株为代表的无致病性马立克氏病病毒。火鸡疱疹病毒及其A变体，以FC126株为代表。对应于第一型病毒株不同致病程度的血清学细分是不可能的。从针对同源和异源试剂的抗原和抗体滴度可以明显看出与3种病毒类型相关的抗原之间的差异。同源抗原和抗体产生的沉淀带比异源试剂产生的沉淀带更强。3种病毒类型的“A”抗原之间的差异以沉淀带的刺突模式为特征，表明存在部分同一性。至少观察到3条“BC”沉淀带；至少1条是组特异性的，1条似乎是型特异性的。
